搜索结果: 'methocult media formulations for human hematopoietic cells serum containing'

重组人IL-34 白细胞介素34，His标签
重组人IL-6 白介素6
重组人PDGF-AA 血小板源性生长因子AA
重组人Persephin Persephin
Lu S-J et al. (JUL 2013) Regenerative medicine 8 4 413--424

3D microcarrier system for efficient differentiation of human pluripotent stem cells into hematopoietic cells without feeders and serum [corrected].

BACKGROUND Human embryonic stem cells (hESCs) have been derived and maintained on mouse embryonic fibroblast feeders to keep their undifferentiated status. To realize their clinical potential,a feeder-free and scalable system for large scale production of hESCs and their differentiated derivatives is required. MATERIALS & METHODS hESCs were cultured and passaged on serum/feeder-free 3D microcarriers for five passages. For embryoid body (EB) formation and hemangioblast differentiation,the medium for 3D microcarriers was directly switched to EB medium. RESULTS hESCs on 3D microcarriers maintained pluripotency and formed EBs,which were ten-times more efficient than hESCs cultured under 2D feeder-free conditions (0.11 ± 0.03 EB cells/hESC input 2D vs 1.19 ± 0.32 EB cells/hESC input 3D). After replating,EB cells from 3D culture readily developed into hemangioblasts with the potential to differentiate into hematopoietic and endothelial cells. Furthermore,this 3D system can also be adapted to human induced pluripotent stem cells,which generate functional hemangioblasts with high efficiency. CONCLUSION This 3D serum- and stromal-free microcarrier system is important for future clinical applications,with the potential of developing to a GMP-compatible scalable system. View Publication

产品类型：

产品号#：

04436

85850

85857

产品名：

MethoCult™ SF H4436

mTeSR™1

mTeSR™1
重组人 CNTF 睫状神经营养因子
人重组网膜蛋白 网膜蛋白
人重组骨桥蛋白 骨桥蛋白，His标签
重组人催乳素 催乳素
人重组抵抗素 抵抗素
重组人TNF - β 肿瘤坏死因子β
重组人TPO 促血小板生成素