搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

文献

Sun AX et al. (AUG 2016) Cell reports 16 7 1942--1953

Direct Induction and Functional Maturation of Forebrain GABAergic Neurons from Human Pluripotent Stem Cells.

Gamma-aminobutyric acid (GABA)-releasing interneurons play an important modulatory role in the cortex and have been implicated in multiple neurological disorders. Patient-derived interneurons could provide a foundation for studying the pathogenesis of these diseases as well as for identifying potential therapeutic targets. Here,we identified a set of genetic factors that could robustly induce human pluripotent stem cells (hPSCs) into GABAergic neurons (iGNs) with high efficiency. We demonstrated that the human iGNs express neurochemical markers and exhibit mature electrophysiological properties within 6-8 weeks. Furthermore,in vitro,iGNs could form functional synapses with other iGNs or with human-induced glutamatergic neurons (iENs). Upon transplantation into immunodeficient mice,human iGNs underwent synaptic maturation and integration into host neural circuits. Taken together,our rapid and highly efficient single-step protocol to generate iGNs may be useful to both mechanistic and translational studies of human interneurons. View Publication

产品类型：

产品号#：

85850

85857

产品名：

mTeSR™1

Event

Austrian Society for Immunology

产品类型：

产品号#：

产品名：

产品手册

Antibodies for Cell Culture

产品类型：

Antibodies

品牌：

EasySep，RosetteSep

产品号#：

60100

60100.1

60100AD

60100AD.1

60108

60108PE

60089

60089AZ

60089AZ.1

60089BT

60089PE

60089PE.1

60091

60091.1

60091AD

60091AD.1

60072

60072.1

60072AD

60072AZ

60072AZ.1

60072BT.1

60072FI

60072FI.1

60072PE

60072PE.1

产品名：

抗β-微管蛋白III抗体，克隆AA10

抗β-微管蛋白III抗体，克隆AA10，Alexa Fluor® 488

小鼠IgG2a，kappa同型对照抗体，克隆C1.18.4

小鼠IgG2a，kappa同型对照抗体，克隆C1.18.4，PE

抗人CD184（CXCR4）抗体，克隆12G5

抗人CD184（CXCR4）抗体，克隆12G5，APC

抗人CD184（CXCR4）抗体，克隆12G5，Biotin

抗人CD184（CXCR4）抗体，克隆12G5，PE

抗人巢蛋白抗体，克隆10C2

抗人巢蛋白抗体，克隆10C2，Alexa Fluor® 488

小鼠 IgG2b，kappa 同型对照抗体（克隆 MPC-11）

小鼠 IgG2b，kappa 同型对照抗体（克隆 MPC-11），Alexa Fluor® 488

小鼠 IgG2b，kappa 同型对照抗体（克隆 MPC-11），APC

小鼠 IgG2b，kappa 同型对照抗体（克隆 MPC-11），Biotin

小鼠 IgG2b，kappa 同型对照抗体（克隆 MPC-11），FITC

小鼠 IgG2b，kappa 同型对照抗体（克隆 MPC-11），PE

文献

A. Reuter et al. ( 2015) The Journal of Immunology 194 2696-2705

Criteria for Dendritic Cell Receptor Selection for Efficient Antibody-Targeted Vaccination

Ab-targeted vaccination involves targeting a receptor of choice expressed by dendritic cells (DCs) with Ag-coupled Abs. Currently,there is little consensus as to which criteria determine receptor selection to ensure superior Ag presentation and immunity. In this study,we investigated parameters of DC receptor internalization and determined how they impact Ag presentation outcomes. First,using mixed bone marrow chimeras,we established that Ag-targeted,but not nontargeted,DCs are responsible for Ag presentation in settings of Ab-targeted vaccination in vivo. Next,we analyzed parameters of DEC205 (CD205),Clec9A,CD11c,CD11b,and CD40 endocytosis and obtained quantitative measurements of internalization speed,surface turnover,and delivered Ag load. Exploiting these parameters in MHC class I (MHC I) and MHC class II (MHC II) Ag presentation assays,we showed that receptor expression level,proportion of surface turnover,or speed of receptor internalization did not impact MHC I or MHC II Ag presentation efficiency. Furthermore,the Ag load delivered to DCs did not correlate with the efficiency of MHC I or MHC II Ag presentation. In contrast,targeting Ag to CD8(+) or CD8(-) DCs enhanced MHC I or MHC II Ag presentation,respectively. Therefore,receptor expression levels,speed of internalization,and/or the amount of Ag delivered can be excluded as major determinants that dictate Ag presentation efficiency in setting of Ab-targeted vaccination. View Publication

产品类型：

产品号#：

19251

产品名：

EasySep™人Pan-DC预富集试剂盒

文献

Wergedal JE et al. (JAN 1992) Metabolism: clinical and experimental 41 1 42--8

Differentiation of normal human bone cells by transforming growth factor-beta and 1,25(OH)2 vitamin D3.

To investigate the role of transforming growth factor-beta 1 (TGF beta) in bone metabolism,the effects of this agent on the differentiation characteristics of human bone cells were studied in vitro. Human bone cells were isolated from femoral head samples by collagenase digestion. Differentiation characteristics included alkaline phosphatase activity,osteocalcin production,and mRNA levels for alkaline phosphatase,type I alpha 2-procollagen,and osteocalcin. The effect of TGF beta on alkaline phosphatase was not constant,but varied with the incubation conditions. At high cell density and in the presence of serum,TGF beta decreased alkaline phosphatase activity. However,at low cell density and under serum-free conditions,TGF beta stimulated alkaline phosphatase activity. The addition of 1,25(OH)2 vitamin D3 also stimulated alkaline phosphatase. The combination of the two agents gave a greater increase in activity than the sum of the activities when the two agents were given alone. The percentage of cells that stain positively for alkaline phosphatase changed in parallel with the change in specific activity. The percentage of positive cells increased from 17% to 64%,while the specific activity increased from 22 to 169 mU/mg protein. To investigate the mechanism of this stimulation,mRNA levels were measured at 24 hours. Individually,TGF beta and 1,25(OH)2D3 increased message levels for alkaline phosphatase and type I procollagen,but the greatest effect was produced by the combination of the two factors. 1,25(OH)2D3 increased osteocalcin mRNA levels,but TGF beta markedly inhibited this stimulation. TGF beta also inhibited production of osteocalcin by the human bone cells. TGF beta appears to modulate differentiation of human bone cells in combination with 1,25(OH)2D3 and other factors. View Publication

产品类型：

产品号#：

72412

产品名：

骨化三醇（Calcitriol）

文献

Balakrishnan K et al. (OCT 2006) Blood 108 7 2392--8

Forodesine, an inhibitor of purine nucleoside phosphorylase, induces apoptosis in chronic lymphocytic leukemia cells.

Purine nucleoside phosphorylase (PNP) deficiency in humans results in T lymphocytopenia. Forodesine,a potent inhibitor of PNP,was designed based on the transition-state structure stabilized by the enzyme. Previous studies established that forodesine in the presence of deoxyguanosine (dGuo) inhibits the proliferation of T lymphocytes. A phase 1 clinical trial of forodesine in T-cell malignancies demonstrated significant antileukemic activity with an increase in intracellular dGuo triphosphate (dGTP). High accumulation of dGTP in T cells may be dependent on the levels of deoxynucleoside kinases. Because B-cell chronic lymphocytic leukemia (B-CLL) cells have high activity of deoxycytidine kinase (dCK),we hypothesized that these lymphocytes would respond to forodesine. This postulate was tested in primary lymphocytes during in vitro investigations. Lymphocytes from 12 patients with CLL were incubated with forodesine and dGuo. These CLL cells showed a wide variation in the accumulation of intracellular dGTP without any effect on other deoxynucleotides. This was associated with DNA damage-induced p53 stabilization,phosphorylation of p53 at Ser15,and activation of p21. The dGTP accumulation was related to induction of apoptosis measured by caspase activation,changes in mitochondrial membrane potential,and PARP cleavage. Based on these data,a phase 2 clinical trial of forodesine has been initiated for CLL patients. View Publication

产品类型：

产品号#：

19051

19051RF

19054

19054RF

产品名：

EasySep™人T细胞富集试剂盒

RoboSep™ 人T细胞富集试剂盒含滤芯吸头

EasySep™人B细胞富集试剂盒

RoboSep™ 人B细胞富集试剂盒含滤芯吸头

文献

Tan J et al. (MAY 2007) Genes & development 21 9 1050--63

Pharmacologic disruption of Polycomb-repressive complex 2-mediated gene repression selectively induces apoptosis in cancer cells.

Polycomb-repressive complex 2 (PRC2)-mediated histone methylation plays an important role in aberrant cancer gene silencing and is a potential target for cancer therapy. Here we show that S-adenosylhomocysteine hydrolase inhibitor 3-Deazaneplanocin A (DZNep) induces efficient apoptotic cell death in cancer cells but not in normal cells. We found that DZNep effectively depleted cellular levels of PRC2 components EZH2,SUZ12,and EED and inhibited associated histone H3 Lys 27 methylation (but not H3 Lys 9 methylation). By integrating RNA interference (RNAi),genome-wide expression analysis,and chromatin immunoprecipitation (ChIP) studies,we have identified a prominent set of genes selectively repressed by PRC2 in breast cancer that can be reactivated by DZNep. We further demonstrate that the preferential reactivation of a set of these genes by DZNep,including a novel apoptosis affector,FBXO32,contributes to DZNep-induced apoptosis in breast cancer cells. Our results demonstrate the unique feature of DZNep as a novel chromatin remodeling compound and suggest that pharmacologic reversal of PRC2-mediated gene repression by DZNep may constitute a novel approach for cancer therapy. View Publication

产品类型：

产品号#：

72322

72324

产品名：

3-Deazaneplanocin A

文献

Brazel CY et al. (AUG 2005) Aging cell 4 4 197--207

Sox2 expression defines a heterogeneous population of neurosphere-forming cells in the adult murine brain.

The identification of neural stem cells (NSCs) in situ has been prevented by the inability to identify a marker consistently expressed in all adult NSCs and is thus generally accomplished using the in vitro neurosphere-forming assay. The high-mobility group transcription factor Sox2 is expressed in embryonic neural epithelial stem cells; because these cells are thought to give rise to the adult NSC population,we hypothesized that Sox2 may continue to be expressed in adult NSCs. Using Sox2:EGFP transgenic mice,we show that Sox2 is expressed in neurogenic regions along the rostral-caudal axis of the central nervous system throughout life. Furthermore,all neurospheres derived from these neurogenic regions express Sox2,suggesting that Sox2 is indeed expressed in adult NSCs. We demonstrate that NSCs are heterogeneous within the adult brain,with differing capacities for cell production. In vitro,all neurospheres express Sox2,but the expression of markers common to early progenitor cells within individual neurospheres varies; this heterogeneity of NSCs is mirrored in vivo. For example,both glial fibrillary acidic protein and NG2 are expressed within individual neurospheres,but their expression is mutually exclusive; likewise,these two markers show distinct staining patterns within the Sox2+ regions of the brain's neurogenic regions. Thus,we propose that the expression of Sox2 is a unifying characteristic of NSCs in the adult brain,but that not all NSCs maintain the ability to form all neural cell types in vivo. View Publication

产品类型：

产品号#：

05700

05701

05702

产品名：

NeuroCult™ 基础培养基（小鼠和大鼠）

NeuroCult™ 扩增添加物（小鼠和大鼠）

NeuroCult™扩增试剂盒（小鼠和大鼠）

文献

Ma N et al. (NOV 2013) Journal of Biological Chemistry 288 48 34671--34679

Transcription activator-like effector nuclease (TALEN)-mediated Gene correction in integration-free β-Thalassemia induced pluripotent stem cells

$\$-Thalassemia ($\$-Thal) is a group of life-threatening blood disorders caused by either point mutations or deletions of nucleotides in $\$-globin gene (HBB). It is estimated that 4.5% of the population in the world carry $\$-Thal mutants (1),posing a persistent threat to public health. The generation of patient-specific induced pluripotent stem cells (iPSCs) and subsequent correction of the disease-causing mutations offer an ideal therapeutic solution to this problem. However,homologous recombination-based gene correction in human iPSCs remains largely inefficient. Here,we describe a robust process combining efficient generation of integration-free $\$-Thal iPSCs from the cells of patients and transcription activator-like effector nuclease (TALEN)-based universal correction of HBB mutations in situ. We generated integration-free and gene-corrected iPSC lines from two patients carrying different types of homozygous mutations and showed that these iPSCs are pluripotent and have normal karyotype. We showed that the correction process did not generate TALEN-induced off targeting mutations by sequencing. More importantly,the gene-corrected $\$-Thal iPS cell lines from each patient can be induced to differentiate into hematopoietic progenitor cells and then further to erythroblasts expressing normal $\$-globin. Our studies provide an efficient and universal strategy to correct different types of $\$-globin mutations in $\$-Thal iPSCs for disease modeling and applications. View Publication

产品类型：

产品号#：

85850

85857

产品名：

mTeSR™1

文献

Cantu' C et al. (JAN 2011) Nucleic acids research 39 2 486--501

A highly conserved SOX6 double binding site mediates SOX6 gene downregulation in erythroid cells.

The Sox6 transcription factor plays critical roles in various cell types,including erythroid cells. Sox6-deficient mice are anemic due to impaired red cell maturation and show inappropriate globin gene expression in definitive erythrocytes. To identify new Sox6 target genes in erythroid cells,we used the known repressive double Sox6 consensus within the εy-globin promoter to perform a bioinformatic genome-wide search for similar,evolutionarily conserved motifs located within genes whose expression changes during erythropoiesis. We found a highly conserved Sox6 consensus within the Sox6 human gene promoter itself. This sequence is bound by Sox6 in vitro and in vivo,and mediates transcriptional repression in transient transfections in human erythroleukemic K562 cells and in primary erythroblasts. The binding of a lentiviral transduced Sox6FLAG protein to the endogenous Sox6 promoter is accompanied,in erythroid cells,by strong downregulation of the endogenous Sox6 transcript and by decreased in vivo chromatin accessibility of this region to the PstI restriction enzyme. These observations suggest that the negative Sox6 autoregulation,mediated by the double Sox6 binding site within its own promoter,may be relevant to control the Sox6 transcriptional downregulation that we observe in human erythroid cultures and in mouse bone marrow cells in late erythroid maturation. View Publication

产品类型：

产品号#：

09600

09650

09850

产品名：

StemSpan™ SFEM

科学海报

Rapid Unbiased Enrichment (Negative Selection) of Circulating Non-Hematopoietic Tumor Cells Directly from Whole Blood

产品类型：

Cell Isolation Products

Conference：

AACR 2012

产品号#：

产品名：

文献

Ortega FJ et al. (FEB 2014) Glia 62 2 247--258

Blockade of microglial K ATP-channel abrogates suppression of inflammatory-mediated inhibition of neural precursor cells

Microglia positively affect neural progenitor cell physiology through the release of inflammatory mediators or trophic factors. We demonstrated previously that reactive microglia foster K(ATP) -channel expression and that blocking this channel using glibenclamide administration enhances striatal neurogenesis after stroke. In this study,we investigated whether the microglial K(ATP) -channel directly influences the activation of neural precursor cells (NPCs) from the subventricular zone using transgenic Csf1r-GFP mice. In vitro exposure of NPCs to lipopolysaccharide and interferon-gamma resulted in a significant decrease in precursor cell number. The complete removal of microglia from the culture or exposure to enriched microglia culture also decreased the precursor cell number. The addition of glibenclamide rescued the negative effects of enriched microglia on neurosphere formation and promoted a 20% improvement in precursor cell number. Similar results were found using microglial-conditioned media from isolated microglia. Using primary mixed glial and pure microglial cultures,glibenclamide specifically targeted reactive microglia to restore neurogenesis and increased the microglial production of the chemokine monocyte chemoattractant protein-1 (MCP-1). These findings provide the first direct evidence that the microglial K(ATP) -channel is a regulator of the proliferation of NPCs under inflammatory conditions. View Publication

产品类型：

产品号#：

05700

05701

05702

产品名：

NeuroCult™ 基础培养基（小鼠和大鼠）

NeuroCult™ 扩增添加物（小鼠和大鼠）

NeuroCult™扩增试剂盒（小鼠和大鼠）

搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

Direct Induction and Functional Maturation of Forebrain GABAergic Neurons from Human Pluripotent Stem Cells.

Criteria for Dendritic Cell Receptor Selection for Efficient Antibody-Targeted Vaccination

Differentiation of normal human bone cells by transforming growth factor-beta and 1,25(OH)2 vitamin D3.

Forodesine, an inhibitor of purine nucleoside phosphorylase, induces apoptosis in chronic lymphocytic leukemia cells.

Pharmacologic disruption of Polycomb-repressive complex 2-mediated gene repression selectively induces apoptosis in cancer cells.

Sox2 expression defines a heterogeneous population of neurosphere-forming cells in the adult murine brain.

Transcription activator-like effector nuclease (TALEN)-mediated Gene correction in integration-free β-Thalassemia induced pluripotent stem cells

A highly conserved SOX6 double binding site mediates SOX6 gene downregulation in erythroid cells.

Blockade of microglial K ATP-channel abrogates suppression of inflammatory-mediated inhibition of neural precursor cells

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