搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

产品手册

Reliable Antibodies for Your Research

产品类型：

品牌：

EasySep

产品号#：

60100

60100.1

60100AD

60100AD.1

60102

60102FI

60104

60104AD

60104AD.1

60104AZ

60104AZ.1

60104BT

60104BT.1

60104BT.2

60104FI

60104FI.1

60104PE

60104PE.1

60106

60106.1

60106AZ

60106AZ.1

60106BT

60106BT.1

60106FI

60106FI.1

60106PE

60106PE.1

60107

60107.1

601

产品名：

抗β-微管蛋白III抗体，克隆AA10

抗β-微管蛋白III抗体，克隆AA10，Alexa Fluor® 488

抗β-微管蛋白III抗体，clone AA10，Alexa Fluor® 488

抗小鼠TCR Gamma/Delta抗体，clone GL3

抗小鼠TCR Gamma/Delta抗体，clone GL3，Alexa Fluor® 488

抗小鼠TCR Gamma/Delta抗体，clone GL3，APC

抗小鼠TCR Gamma/Delta抗体，clone GL3，Biotin

抗小鼠TCR Gamma/Delta抗体，clone GL3，FITC

抗小鼠TCR Gamma/Delta抗体，clone GL3，PE

抗人CD71（转铁蛋白受体）抗体，clone OKT9

抗人CD71（转铁蛋白受体）抗体，clone OKT9，APC

抗人CD71（转铁蛋白受体）抗体，clone OKT9，Biotin

抗人CD71（转铁蛋白受体）抗体，clone OKT9，FITC

抗人CD71（转铁蛋白受体）抗体，clone OKT9，PE

产品手册

qPCR Arrays for Cell Characterization

产品类型：

品牌：

MesenCult

产品号#：

05110

05115

05220

05221

05401

05402

05411

05412

05455

05910

05916

05946

05230

05970

05120

03950

05240

85850

85857

85870

85875

05270

05275

05271

05439

05990

07515

07516

07517

07521

07522

07523

07524

07531

07532

07533

07534

07541

07542

07543

07544

05876

055

产品名：

STEMdiff™定型内胚层检测试剂盒

STEMdiff™ 中胚层诱导培养基

MesenCult™ MSC 基础培养基（人）

MesenCult™ MSC刺激添加物（人）

MesenCult™ 增殖试剂盒（人）

MesenCult™ 脂肪分化试剂盒 (人)

MesenCult™-ACF软骨细胞分化试剂盒

TeSR™-E5

TeSR™-E6

STEMdiff™ 三胚层分化试剂盒

STEMdiff™胰腺祖细胞试剂盒

mTeSR™3D

STEMdiff™ 间充质祖细胞试剂盒

mTeSR™1

STEMdiff™ APEL™2 培养基

MesenCult™-hPL 培养基试剂盒

用于hESC/hiPSC维持培养的TeSR™-E8™

人多能干细胞三谱系分化qPCR阵列

qPCR预混试剂盒

Naïve态人多能干细胞 qPCR 阵列

人间充质干细胞 qPCR 阵列分析

不含酚红的mTeSR™1

发布日期: 05/02/2018

技术手册

Enzymatic Dissociation of Adult Mouse and Rat CNS Tissue Using the NeuroCult™ Enzymatic Dissociation Kit

产品类型：

产品号#：

05715

产品名：

NeuroCult™成年中枢神经系统（CNS）组织酶解试剂盒（小鼠和大鼠）

科学海报

CRISPR-Cas9 Gene Editing Of CDK5RAP2 In Human Pluripotent Stem Cells, Derivation Of Genetically Stable Clonal Lines And Formation Of Cerebral Organoids

产品类型：

Conference：

EMBL Organoids 2018

产品号#：

08570

08571

产品名：

STEMdiff™ 脑类器官试剂盒

STEMdiff™ 脑类器官成熟试剂盒

Barrett LE et al. (JAN 2012) Cancer cell 21 1 11--24

Self-renewal does not predict tumor growth potential in mouse models of high-grade glioma.

Within high-grade gliomas,the precise identities and functional roles of stem-like cells remain unclear. In the normal neurogenic niche,ID (Inhibitor of DNA-binding) genes maintain self-renewal and multipotency of adult neural stem cells. Using PDGF- and KRAS-driven murine models of gliomagenesis,we show that high Id1 expression (Id1(high)) identifies tumor cells with high self-renewal capacity,while low Id1 expression (Id1(low)) identifies tumor cells with proliferative potential but limited self-renewal capacity. Surprisingly,Id1(low) cells generate tumors more rapidly and with higher penetrance than Id1(high) cells. Further,eliminating tumor cell self-renewal through deletion of Id1 has modest effects on animal survival,while knockdown of Olig2 within Id1(low) cells has a significant survival benefit,underscoring the importance of non-self-renewing lineages in disease progression. View Publication

产品类型：

产品号#：

05700

05701

05702

产品名：

NeuroCult™ 基础培养基（小鼠和大鼠）

NeuroCult™ 扩增添加物（小鼠和大鼠）

NeuroCult™扩增试剂盒（小鼠和大鼠）

Udagawa J et al. (FEB 2006) Endocrinology 147 2 647--58

The role of leptin in the development of the cerebral cortex in mouse embryos.

Leptin is detected in the sera,and leptin receptors are expressed in the cerebrum of mouse embryos,suggesting that leptin plays a role in cerebral development. Compared with the wild type,leptin-deficient (ob/ob) mice had fewer cells at embryonic day (E) 16 and E18 and had fewer 5-bromo-2'-deoxyuridine(+) cells at E14 and E16 in the neuroepithelium. Intracerebroventricular leptin injection in E14 ob/ob embryos increased the number of neuroepithelium cells at E16. In cultured neurosphere cells,leptin treatment increased Hes1 mRNA expression and maintained neural progenitors. Astrocyte differentiation was induced by low-dose (0.1 microg/ml) but not high-dose (1 microg/ml) leptin. High-dose leptin decreased Id mRNA and increased Ngn1 mRNA in neurosphere cells. The neuropeptide Y mRNA level in the cortical plate was lower in ob/ob than the wild type at E16 and E18. These results suggest that leptin maintains neural progenitors and is related to glial and neuronal development in embryos. View Publication

产品类型：

产品号#：

05700

05701

05702

05703

05704

产品名：

NeuroCult™ 基础培养基（小鼠和大鼠）

NeuroCult™ 扩增添加物（小鼠和大鼠）

NeuroCult™扩增试剂盒（小鼠和大鼠）

NeuroCult™ 分化添加物（小鼠和大鼠）

NeuroCult™ 分化试剂盒（小鼠和大鼠）

Kishigami S et al. (FEB 2006) Biochemical and biophysical research communications 340 1 183--9

Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer.

The low success rate of animal cloning by somatic cell nuclear transfer (SCNT) is believed to be associated with epigenetic errors including abnormal DNA hypermethylation. Recently,we elucidated by using round spermatids that,after nuclear transfer,treatment of zygotes with trichostatin A (TSA),an inhibitor of histone deacetylase,can remarkably reduce abnormal DNA hypermethylation depending on the origins of transferred nuclei and their genomic regions [S. Kishigami,N. Van Thuan,T. Hikichi,H. Ohta,S. Wakayama. E. Mizutani,T. Wakayama,Epigenetic abnormalities of the mouse paternal zygotic genome associated with microinsemination of round spermatids,Dev. Biol. (2005) in press]. Here,we found that 5-50 nM TSA-treatment for 10 h following oocyte activation resulted in more efficient in vitro development of somatic cloned embryos to the blastocyst stage from 2- to 5-fold depending on the donor cells including tail tip cells,spleen cells,neural stem cells,and cumulus cells. This TSA-treatment also led to more than 5-fold increase in success rate of mouse cloning from cumulus cells without obvious abnormality but failed to improve ES cloning success. Further,we succeeded in establishment of nuclear transfer-embryonic stem (NT-ES) cells from TSA-treated cloned blastocyst at a rate three times higher than those from untreated cloned blastocysts. Thus,our data indicate that TSA-treatment after SCNT in mice can dramatically improve the practical application of current cloning techniques. View Publication

产品类型：

产品号#：

05700

05701

05702

72282

72284

产品名：

NeuroCult™ 基础培养基（小鼠和大鼠）

NeuroCult™ 扩增添加物（小鼠和大鼠）

NeuroCult™扩增试剂盒（小鼠和大鼠）

曲古抑菌素 A（Trichostatin A）

科学海报

A Reliable, Efficient, and Feeder-Free Method to Generate Brain-Region-Specific Dorsal and Ventral Forebrain Organoids From Human Pluripotent Stem Cells to Model Early Human Brain Development

产品类型：

Conference：

CSHL 3D Brain 2019

产品号#：

产品名：

发布日期: 12/20/2019

Chailangkarn T et al. (AUG 2016) Nature 536 7616 338--343

A human neurodevelopmental model for Williams syndrome.

Williams syndrome is a genetic neurodevelopmental disorder characterized by an uncommon hypersociability and a mosaic of retained and compromised linguistic and cognitive abilities. Nearly all clinically diagnosed individuals with Williams syndrome lack precisely the same set of genes,with breakpoints in chromosome band 7q11.23 (refs 1-5). The contribution of specific genes to the neuroanatomical and functional alterations,leading to behavioural pathologies in humans,remains largely unexplored. Here we investigate neural progenitor cells and cortical neurons derived from Williams syndrome and typically developing induced pluripotent stem cells. Neural progenitor cells in Williams syndrome have an increased doubling time and apoptosis compared with typically developing neural progenitor cells. Using an individual with atypical Williams syndrome,we narrowed this cellular phenotype to a single gene candidate,frizzled 9 (FZD9). At the neuronal stage,layer V/VI cortical neurons derived from Williams syndrome were characterized by longer total dendrites,increased numbers of spines and synapses,aberrant calcium oscillation and altered network connectivity. Morphometric alterations observed in neurons from Williams syndrome were validated after Golgi staining of post-mortem layer V/VI cortical neurons. This model of human induced pluripotent stem cells fills the current knowledge gap in the cellular biology of Williams syndrome and could lead to further insights into the molecular mechanism underlying the disorder and the human social brain. View Publication

产品类型：

产品号#：

05850

05857

05870

05875

85850

85857

85870

85875

产品名：

mTeSR™1

技术手册

Chemical Dissociation of Neurospheres Derived from Embryonic and Adult Mouse CNS using the NeuroCult™ Chemical Dissociation Kit

产品类型：

产品号#：

05707

产品名：

NeuroCult™化学解离试剂盒（小鼠）

Yamamizu K et al. (DEC 2013) Stem Cell Reports 1 6 545--559

Identification of Transcription Factors for Lineage-Specific ESC Differentiation

A network of transcription factors (TFs) determines cell identity,but identity can be altered by overexpressing a combination of TFs. However,choosing and verifying combinations of TFs for specific cell differentiation have been daunting due to the large number of possible combinations of 2,000 TFs. Here,we report the identification of individual TFs for lineage-specific cell differentiation based on the correlation matrix of global gene expression profiles. The overexpression of identified TFs-Myod1,Mef2c,Esx1,Foxa1,Hnf4a,Gata2,Gata3,Myc,Elf5,Irf2,Elf1,Sfpi1,Ets1,Smad7,Nr2f1,Sox11,Dmrt1,Sox9,Foxg1,Sox2,or Ascl1-can direct efficient,specific,and rapid differentiation into myocytes,hepatocytes,blood cells,and neurons. Furthermore,transfection of synthetic mRNAs of TFs generates their appropriate target cells. These results demonstrate both the utility of this approach to identify potent TFs for cell differentiation,and the unanticipated capacity of single TFs directly guides differentiation to specific lineage fates. View Publication

产品类型：

产品号#：

05700

05704

产品名：

NeuroCult™ 基础培养基（小鼠和大鼠）

NeuroCult™ 分化试剂盒（小鼠和大鼠）

Lee SB et al. (JAN 2016) Nature 529 7585 172--7

An ID2-dependent mechanism for VHL inactivation in cancer.

Mechanisms that maintain cancer stem cells are crucial to tumour progression. The ID2 protein supports cancer hallmarks including the cancer stem cell state. HIFα transcription factors,most notably HIF2α (also known as EPAS1),are expressed in and required for maintenance of cancer stem cells (CSCs). However,the pathways that are engaged by ID2 or drive HIF2α accumulation in CSCs have remained unclear. Here we report that DYRK1A and DYRK1B kinases phosphorylate ID2 on threonine 27 (Thr27). Hypoxia downregulates this phosphorylation via inactivation of DYRK1A and DYRK1B. The activity of these kinases is stimulated in normoxia by the oxygen-sensing prolyl hydroxylase PHD1 (also known as EGLN2). ID2 binds to the VHL ubiquitin ligase complex,displaces VHL-associated Cullin 2,and impairs HIF2α ubiquitylation and degradation. Phosphorylation of Thr27 of ID2 by DYRK1 blocks ID2-VHL interaction and preserves HIF2α ubiquitylation. In glioblastoma,ID2 positively modulates HIF2α activity. Conversely,elevated expression of DYRK1 phosphorylates Thr27 of ID2,leading to HIF2α destabilization,loss of glioma stemness,inhibition of tumour growth,and a more favourable outcome for patients with glioblastoma. View Publication

产品类型：

产品号#：

05700

05701

05702

产品名：

NeuroCult™ 基础培养基（小鼠和大鼠）

NeuroCult™ 扩增添加物（小鼠和大鼠）

NeuroCult™扩增试剂盒（小鼠和大鼠）

搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

Self-renewal does not predict tumor growth potential in mouse models of high-grade glioma.

The role of leptin in the development of the cerebral cortex in mouse embryos.

Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer.

A human neurodevelopmental model for Williams syndrome.

Identification of Transcription Factors for Lineage-Specific ESC Differentiation

An ID2-dependent mechanism for VHL inactivation in cancer.

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