搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

实验方案

How to Tri-Culture Astrocytes, Microglia, and NGN2 mRNA-LNP-Induced Forebrain Neurons Derived from Human Pluripotent Stem Cells

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研究方向：

疾病建模，神经科学，药物发现和毒理检测

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产品名：

Chua SJ et al. (FEB 2009) Biochemical and biophysical research communications 379 2 217--21

Neural progenitors, neurons and oligodendrocytes from human umbilical cord blood cells in a serum-free, feeder-free cell culture.

We have previously demonstrated that lineage negative cells (Lin(neg)) from umbilical cord blood (UCB) develop into multipotent cells capable of differentiation into bone,muscle,endothelial and neural cells. The objective of this study was to determine the optimal conditions required for Lin(neg) UCB cells to differentiate into neuronal cells and oligodendrocytes. We demonstrate that early neural stage markers (nestin,neurofilament,A2B5 and Sox2) are expressed in Lin(neg) cells cultured in FGF4,SCF,Flt3-ligand reprogramming culture media followed by the early macroglial cell marker O4. Early stage oligodendrocyte markers CNPase,GalC,Olig2 and the late-stage marker MOSP are observed,as is the Schwann cell marker PMP22. In summary,Lin(neg) UCB cells,when appropriately cultured,are able to exhibit characteristics of neuronal and macroglial cells that can specifically differentiate into oligodendrocytes and Schwann cells and express proteins associated with myelin production after in vitro differentiation. View Publication

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产品号#：

09600

09650

产品名：

StemSpan™ SFEM

Vukovic J et al. (APR 2013) Journal of Neuroscience 33 15 6603--6613

Immature Doublecortin-Positive Hippocampal Neurons Are Important for Learning But Not for Remembering

It is now widely accepted that hippocampal neurogenesis underpins critical cognitive functions,such as learning and memory. To assess the behavioral importance of adult-born neurons,we developed a novel knock-in mouse model that allowed us to specifically and reversibly ablate hippocampal neurons at an immature stage. In these mice,the diphtheria toxin receptor (DTR) is expressed under control of the doublecortin (DCX) promoter,which allows for specific ablation of immature DCX-expressing neurons after administration of diphtheria toxin while leaving the neural precursor pool intact. Using a spatially challenging behavioral test (a modified version of the active place avoidance test),we present direct evidence that immature DCX-expressing neurons are required for successful acquisition of spatial learning,as well as reversal learning,but are not necessary for the retrieval of stored long-term memories. Importantly,the observed learning deficits were rescued as newly generated immature neurons repopulated the granule cell layer upon termination of the toxin treatment. Repeat (or cyclic) depletion of immature neurons reinstated behavioral deficits if the mice were challenged with a novel task. Together,these findings highlight the potential of stimulating neurogenesis as a means to enhance learning. View Publication

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产品号#：

05700

05701

05702

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NeuroCult™ 基础培养基(小鼠&大鼠)

NeuroCult™ 扩增添加物 (小鼠&大鼠)

NeuroCult™ 扩增试剂盒 (小鼠&大鼠)

科学海报

The Downstream Differentiation Potential of Human Induced Pluripotent Stem Cell-Derived Neural Progenitor Cells to Forebrain Neurons and Astrocytes

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Conference：

SfN 2023

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产品名：

Yamamizu K et al. (MAY 2016) Scientific reports 6 1 25667

Generation and gene expression profiling of 48 transcription-factor-inducible mouse embryonic stem cell lines.

Mouse embryonic stem cells (ESCs) can differentiate into a wide range - and possibly all cell types in vitro,and thus provide an ideal platform to study systematically the action of transcription factors (TFs) in cell differentiation. Previously,we have generated and analyzed 137 TF-inducible mouse ESC lines. As an extension of this NIA Mouse ESC Bank we generated and characterized 48 additional mouse ESC lines,in which single TFs in each line could be induced in a doxycycline-controllable manner. Together,with the previous ESC lines,the bank now comprises 185 TF-manipulable ESC lines (>10% of all mouse TFs). Global gene expression (transcriptome) profiling revealed that the induction of individual TFs in mouse ESCs for 48 hours shifts their transcriptomes toward specific differentiation fates (e.g.,neural lineages by Myt1 Isl1,and St18; mesodermal lineages by Pitx1,Pitx2,Barhl2,and Lmx1a; white blood cells by Myb,Etv2,and Tbx6,and ovary by Pitx1,Pitx2,and Dmrtc2). These data also provide and lists of inferred target genes of each TF and possible functions of these TFs. The results demonstrate the utility of mouse ESC lines and their transcriptome data for understanding the mechanism of cell differentiation and the function of TFs. View Publication

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产品号#：

05700

05703

05704

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NeuroCult™ 基础培养基(小鼠&大鼠)

NeuroCult™ 分化添加物 (小鼠&大鼠)

NeuroCult™ 分化试剂盒 (小鼠&大鼠)

产品手册

qPCR Arrays for Cell Characterization

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品牌：

MesenCult

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05110

05115

05220

05221

05401

05402

05411

05412

05455

05910

05916

05946

05230

05970

05120

03950

05240

85850

85857

85870

85875

05270

05275

05271

05439

05990

07515

07516

07517

07521

07522

07523

07524

07531

07532

07533

07534

07541

07542

07543

07544

05876

055

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STEMdiff™定型内胚层检测试剂盒

STEMdiff™ 中胚层诱导培养基

MesenCult™ MSC基础培养基 (人)

MesenCult™ MSC 刺激补充剂（人）

MesenCult™ 增殖试剂盒（人）

MesenCult™ 脂肪分化试剂盒 (人)

MesenCult™-ACF软骨细胞分化试剂盒

TeSR™-E5

TeSR™-E6

STEMdiff™ 三谱系分化试剂盒

STEMdiff™胰腺祖细胞试剂盒

mTeSR™3D

STEMdiff™ 间充质祖细胞试剂盒

mTeSR™1

STEMdiff™ APEL™2 培养基

MesenCult™-hPL 培养基试剂盒

TeSR™-E8™

人多能干细胞三谱系分化qPCR阵列

qPCR预混试剂盒

Naïve态人多能干细胞 qPCR 阵列

人间充质干细胞 qPCR 阵列分析

不含酚红的mTeSR™1

Bull ND and Bartlett PF (NOV 2005) The Journal of neuroscience : the official journal of the Society for Neuroscience 25 47 10815--21

The adult mouse hippocampal progenitor is neurogenic but not a stem cell.

The aim of this investigation was to characterize the proliferative precursor cells in the adult mouse hippocampal region. Given that a very large number of new hippocampal cells are generated over the lifetime of an animal,it is predicted that a neural stem cell is ultimately responsible for maintaining this genesis. Although it is generally accepted that a proliferative precursor resides within the hippocampus,contradictory reports exist regarding the classification of this cell. Is it a true stem cell or a more limited progenitor? Using a strict functional definition of a neural stem cell and a number of in vitro assays,we report that the resident hippocampal precursor is a progenitor capable of proliferation and multipotential differentiation but is unable to self-renew and thus proliferate indefinitely. Furthermore,the mitogen FGF-2 stimulates proliferation of these cells to a greater extent than epidermal growth factor (EGF). In addition,we found that BDNF was essential for the production of neurons from the hippocampal progenitor cells,being required during proliferation to trigger neuronal fate. In contrast,a bona fide neural stem cell was identified in the lateral wall of the lateral ventricle surrounding the hippocampus. Interestingly,EGF proved to be the stronger mitogenic factor for this cell,which was clearly a different precursor from the resident hippocampal progenitor. These results suggest that the stem cell ultimately responsible for adult hippocampal neurogenesis resides outside the hippocampus,producing progenitor cells that migrate into the neurogenic zones and proliferate to produce new neurons and glia. View Publication

产品类型：

产品号#：

05700

05701

05702

05740

产品名：

NeuroCult™ 基础培养基(小鼠&大鼠)

NeuroCult™ 扩增添加物 (小鼠&大鼠)

NeuroCult™ 扩增试剂盒 (小鼠&大鼠)

Foti SB et al. (OCT 2013) International Journal of Developmental Neuroscience 31 6 434--447

HDAC inhibitors dysregulate neural stem cell activity in the postnatal mouse brain

The mammalian central nervous system (CNS) undergoes significant expansion postnatally,producing astrocytes,oligodendrocytes and inhibitory neurons to modulate the activity of neural circuits. This is coincident in humans with the emergence of pediatric epilepsy,a condition commonly treated with valproate/valproic acid (VPA),a potent inhibitor of histone deacetylases (HDACs). The sequential activity of specific HDACs,however,may be essential for the differentiation of distinct subpopulations of neurons and glia. Here,we show that different subsets of CNS neural stem cells (NSCs) and progenitors switch expression of HDAC1 and HDAC2 as they commit to a neurogenic lineage in the subventricular zone (SVZ) and dentate gyrus (DG). The administration of VPA for only one week from P7-P14,combined with sequential injections of thymidine analogs reveals that VPA stimulates a significant and differential decrease in the production and differentiation of progeny of NSCs in the DG,rostral migratory stream (RMS),and olfactory bulb (OB). Cross-fostering VPA-treated mice revealed,however,that a postnatal failure to thrive induced by VPA treatment had a greater effect on DG neurogenesis than VPA action directly. By one month after VPA,OB interneuron genesis was significantly and differentially reduced in both periglomerular and granule neurons. Using neurosphere assays to test if VPA directly regulates NSC activity,we found that short term treatment with VPA in vivo reduced neurosphere numbers and size,a phenotype that was also obtained in neurospheres from control mice treated with VPA and an alternative HDAC inhibitor,Trichostatin A (TSA) at 0 and 3 days in vitro (DIV). Collectively,these data show that clinically used HDAC inhibitors like VPA and TSA can perturb postnatal neurogenesis; and their use should be carefully considered,especially in individuals whose brains are actively undergoing key postnatal time windows of development. View Publication

产品类型：

产品号#：

05700

05701

05702

产品名：

NeuroCult™ 基础培养基(小鼠&大鼠)

NeuroCult™ 扩增添加物 (小鼠&大鼠)

NeuroCult™ 扩增试剂盒 (小鼠&大鼠)

技术手册

Identification and Enumeration of Rat Neural Stem and Progenitor Cells With the NeuroCult™ Neural Colony-Forming Cell (NCFC) Assay Kit

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产品号#：

05742

产品名：

产品手册

Reliable Antibodies for Your Research

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品牌：

EasySep

产品号#：

60100

60100.1

60100AD

60100AD.1

60102

60102FI

60104

60104AD

60104AD.1

60104AZ

60104AZ.1

60104BT

60104BT.1

60104BT.2

60104FI

60104FI.1

60104PE

60104PE.1

60106

60106.1

60106AZ

60106AZ.1

60106BT

60106BT.1

60106FI

60106FI.1

60106PE

60106PE.1

60107

60107.1

601

产品名：

抗β-微管蛋白III抗体，克隆AA10

抗β-微管蛋白III抗体，克隆AA10，Alexa Fluor® 488

抗β-微管蛋白III抗体，clone AA10，Alexa Fluor® 488

抗小鼠TCR Gamma/Delta抗体，clone GL3

抗小鼠TCR Gamma/Delta抗体，clone GL3，Alexa Fluor® 488

抗小鼠TCR Gamma/Delta抗体，clone GL3，APC

抗小鼠TCR Gamma/Delta抗体，clone GL3，Biotin

抗小鼠TCR Gamma/Delta抗体，clone GL3，FITC

抗小鼠TCR Gamma/Delta抗体，clone GL3，PE

抗人CD71（转铁蛋白受体）抗体，clone OKT9

抗人CD71（转铁蛋白受体）抗体，clone OKT9，APC

抗人CD71（转铁蛋白受体）抗体，clone OKT9，Biotin

抗人CD71（转铁蛋白受体）抗体，clone OKT9，FITC

抗人CD71（转铁蛋白受体）抗体，clone OKT9，PE

技术手册

Enzymatic Dissociation of Adult Mouse and Rat CNS Tissue Using the NeuroCult™ Enzymatic Dissociation Kit

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产品号#：

05715

产品名：

NeuroCult™成年中枢神经系统（CNS）组织酶解试剂盒（小鼠和大鼠）

科学海报

CRISPR-Cas9 Gene Editing Of CDK5RAP2 In Human Pluripotent Stem Cells, Derivation Of Genetically Stable Clonal Lines And Formation Of Cerebral Organoids

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Conference：

EMBL Organoids 2018

产品号#：

08570

08571

产品名：

STEMdiff™ 脑类器官试剂盒

STEMdiff™ 脑类器官成熟试剂盒

搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

Neural progenitors, neurons and oligodendrocytes from human umbilical cord blood cells in a serum-free, feeder-free cell culture.

Immature Doublecortin-Positive Hippocampal Neurons Are Important for Learning But Not for Remembering

Generation and gene expression profiling of 48 transcription-factor-inducible mouse embryonic stem cell lines.

The adult mouse hippocampal progenitor is neurogenic but not a stem cell.

HDAC inhibitors dysregulate neural stem cell activity in the postnatal mouse brain

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