搜索结果: 'gmp t cell activator'

科学海报

A Reliable, Efficient, and Matrix-Free Method to Generate Midbrain Organoids from Human Pluripotent Stem Cells

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Conference：

CSHL 3D Brain 2022

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STEMCELL Journal Club: Cerebral Organoids for Human-Specific Infection Modeling

28:45

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STEMCELL Journal Club: Cerebral Organoids for Human-Specific Infection Modeling

发布日期: 05/25/2020

Modeling Genetic Epilepsies with Induced Pluripotent Stem Cells

53:16:00

视频

Modeling Genetic Epilepsies with Induced Pluripotent Stem Cells

发布日期: 06/01/2017

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STEMCELL 神经科学研究工具

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BrainPhys

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05702

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08605

05796

100-0019

100-0020

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100-0038

100-0041

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NeuroCult™扩增试剂盒（小鼠和大鼠）

NeuroCult™ 分化试剂盒（小鼠和大鼠）

NeuroCult™ SM1 神经添加物

NeuroCult™ NS-A 基础培养基（人）

NeuroCult™ NS-A 扩增试剂盒（人）

STEMdiff™ 神经花环选择试剂

STEMdiff™神经前体细胞培养基

BrainPhys™神经元培养基

BrainPhys™神经元培养基和SM1试剂盒

BrainPhys™ 神经元培养基N2-A和SM1试剂盒

BrainPhys™原代神经元试剂盒

BrainPhys™ hPSC 神经元试剂盒

BrainPhys™ 无酚红

STEMdiff™ 脑类器官试剂盒

STEMdiff™ 脑类器官成熟试剂盒

STEMdiff™ 神经诱导培养基

qPCR预混试剂盒

STEMdiff™SMADi神经诱导试剂盒

STEMdiff™SMADi神经诱导试剂盒，2套

STEMdiff™ 神经嵴分化试剂盒

STEMdiff™前脑神经元分化试剂盒

STEMdiff™ 前脑神经元成熟试剂盒

BrainPhys™成像专用培养基

STEMdiff™ 小胶质细胞分化试剂盒

STEMdiff™ 小胶质细胞成熟试剂盒

STEMdiff™ 星形胶质细胞分化试剂盒

STEMdiff™ 星形胶质细胞成熟试剂盒

STEMdiff™中脑神经元分化试剂盒

STEMdiff™ 中脑神经元成熟试剂盒

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Modeling Neuroinflammation with hPSC-Derived Cells

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STEMdiff

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科学海报

Expansion of Rat Neural Stem and Progenitor Cells in Long-Term Culture

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Conference：

SFN 2005

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05701

05702

05740

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05771

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NeuroCult™ 扩增添加物（小鼠和大鼠）

NeuroCult™扩增试剂盒（小鼠和大鼠）

技术手册

Generation and Culture of Neural Progenitor Cells using the STEMdiff™ Neural System

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00219

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STEMdiff™ 神经花环选择试剂

STEMdiff™神经前体细胞培养基

STEMdiff™ 神经诱导培养基

STEMdiff™SMADi神经诱导试剂盒

STEMdiff™SMADi神经诱导试剂盒，2套

技术手册

In Vitro Proliferation and Differentiation of Mouse and Rat Neural Stem and Progenitor Cells Using NeuroCult™

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05700

05701

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NeuroCult™ 基础培养基（小鼠和大鼠）

NeuroCult™ 扩增添加物（小鼠和大鼠）

NeuroCult™扩增试剂盒（小鼠和大鼠）

NeuroCult™ 分化添加物（小鼠和大鼠）

NeuroCult™ 分化试剂盒（小鼠和大鼠）

技术手册

In Vitro Proliferation and Differentiation of Human Neural Stem and Progenitor Cells using NeuroCult™ or NeuroCult™-XF

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05751

05752

05761

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NeuroCult™ NS-A 扩增试剂盒（人）

NeuroCult™ NS-A 分化试剂盒（人）

用于小鼠和大鼠神经干细胞和祖细胞分化培养的试剂盒

Lee S-HH et al. (JUN 2000) Nature biotechnology 18 6 675--9

Efficient generation of midbrain and hindbrain neurons from mouse embryonic stem cells.

Embryonic stem (ES) cells are clonal cell lines derived from the inner cell mass of the developing blastocyst that can proliferate extensively in vitro and are capable of adopting all the cell fates in a developing embryo. Clinical interest in the use of ES cells has been stimulated by studies showing that isolated human cells with ES properties from the inner cell mass or developing germ cells can provide a source of somatic precursors. Previous studies have defined in vitro conditions for promoting the development of specific somatic fates,specifically,hematopoietic,mesodermal,and neurectodermal. In this study,we present a method for obtaining dopaminergic (DA) and serotonergic neurons in high yield from mouse ES cells in vitro. Furthermore,we demonstrate that the ES cells can be obtained in unlimited numbers and that these neuron types are generated efficiently. We generated CNS progenitor populations from ES cells,expanded these cells and promoted their differentiation into dopaminergic and serotonergic neurons in the presence of mitogen and specific signaling molecules. The differentiation and maturation of neuronal cells was completed after mitogen withdrawal from the growth medium. This experimental system provides a powerful tool for analyzing the molecular mechanisms controlling the functions of these neurons in vitro and in vivo,and potentially for understanding and treating neurodegenerative and psychiatric diseases. View Publication

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06902

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N2 添加物-A

Zhou Y et al. (DEC 2016) Molecular autism 7 1 42

CGG-repeat dynamics and FMR1 gene silencing in fragile X syndrome stem cells and stem cell-derived neurons.

BACKGROUND Fragile X syndrome (FXS),a common cause of intellectual disability and autism,results from the expansion of a CGG-repeat tract in the 5' untranslated region of the FMR1 gene to<200 repeats. Such expanded alleles,known as full mutation (FM) alleles,are epigenetically silenced in differentiated cells thus resulting in the loss of FMRP,a protein important for learning and memory. The timing of repeat expansion and FMR1 gene silencing is controversial. METHODS We monitored the repeat size and methylation status of FMR1 alleles with expanded CGG repeats in patient-derived induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) that were grown for extended period of time either as stem cells or differentiated into neurons. We used a PCR assay optimized for the amplification of large CGG repeats for sizing,and a quantitative methylation-specific PCR for the analysis of FMR1 promoter methylation. The FMR1 mRNA levels were analyzed by qRT-PCR. FMRP levels were determined by western blotting and immunofluorescence. Chromatin immunoprecipitation was used to study the association of repressive histone marks with the FMR1 gene in FXS ESCs. RESULTS We show here that while FMR1 gene silencing can be seen in FXS embryonic stem cells (ESCs),some silenced alleles contract and when the repeat number drops below ˜400,DNA methylation erodes,even when the repeat number remains<200. The resultant active alleles do not show the large step-wise expansions seen in stem cells from other repeat expansion diseases. Furthermore,there may be selection against large active alleles and these alleles do not expand further or become silenced on neuronal differentiation. CONCLUSIONS Our data support the hypotheses that (i) large expansions occur prezygotically or in the very early embryo,(ii) large unmethylated alleles may be deleterious in stem cells,(iii) methylation can occur on alleles with<400 repeats very early in embryogenesis,and (iv) expansion and contraction may occur by different mechanisms. Our data also suggest that the threshold for stable methylation of FM alleles may be higher than previously thought. A higher threshold might explain why some carriers of FM alleles escape methylation. It may also provide a simple explanation for why silencing has not been observed in mouse models with<200 repeats. View Publication

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05832

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STEMdiff™ 神经花环选择试剂

mTeSR™1

Pollak J et al. (MAR 2017) PLOS ONE 12 3 e0172884

Ion channel expression patterns in glioblastoma stem cells with functional and therapeutic implications for malignancy

Ion channels and transporters have increasingly recognized roles in cancer progression through the regulation of cell proliferation,migration,and death. Glioblastoma stem-like cells (GSCs) are a source of tumor formation and recurrence in glioblastoma multiforme,a highly aggressive brain cancer,suggesting that ion channel expression may be perturbed in this population. However,little is known about the expression and functional relevance of ion channels that may contribute to GSC malignancy. Using RNA sequencing,we assessed the enrichment of ion channels in GSC isolates and non-tumor neural cell types. We identified a unique set of GSC-enriched ion channels using differential expression analysis that is also associated with distinct gene mutation signatures. In support of potential clinical relevance,expression of selected GSC-enriched ion channels evaluated in human glioblastoma databases of The Cancer Genome Atlas and Ivy Glioblastoma Atlas Project correlated with patient survival times. Finally,genetic knockdown as well as pharmacological inhibition of individual or classes of GSC-enriched ion channels constrained growth of GSCs compared to normal neural stem cells. This first-in-kind global examination characterizes ion channels enriched in GSCs and explores their potential clinical relevance to glioblastoma molecular subtypes,gene mutations,survival outcomes,regional tumor expression,and experimental responses to loss-of-function. Together,the data support the potential biological and therapeutic impact of ion channels on GSC malignancy and provide strong rationale for further examination of their mechanistic and therapeutic importance. View Publication

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05751

70913

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NeuroCult™ NS-A 扩增试剂盒（人）

搜索结果: 'gmp t cell activator'

Efficient generation of midbrain and hindbrain neurons from mouse embryonic stem cells.

CGG-repeat dynamics and FMR1 gene silencing in fragile X syndrome stem cells and stem cell-derived neurons.

Ion channel expression patterns in glioblastoma stem cells with functional and therapeutic implications for malignancy

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