搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

产品手册

ProstaCult™: Detect 7 Times More Mouse Prostate Epithelial Progenitors

产品类型：

品牌：

ProstaCult

产品号#：

02633

02634

02654

05640

07900

07901

07912

07913

27305

19758

78016

78016.1

78108

78108.1

产品名：

DNase I 溶液（1 mg/mL）

胰蛋白酶-EDTA (0.25%)

胶原酶/透明质酸酶

Dispase（5 U/mL）

重组小鼠 EGF

重组小鼠FGF-21

Growth of Mouse Pancreatic Organoids in PancreaCult™ Culture Medium: Under a Microscope

1:07

视频

Growth of Mouse Pancreatic Organoids in PancreaCult™ Culture Medium: Under a Microscope

发布日期: 4/18/18

文献

Charafe-Jauffret E et al. (JAN 2010) Clinical cancer research : an official journal of the American Association for Cancer Research 16 1 45--55

Aldehyde dehydrogenase 1-positive cancer stem cells mediate metastasis and poor clinical outcome in inflammatory breast cancer.

PURPOSE: To examine the role of cancer stem cells (CSC) in mediating metastasis in inflammatory breast cancer (IBC) and the association of these cells with patient outcome in this aggressive type of breast cancer. EXPERIMENTAL DESIGN: CSCs were isolated from SUM149 and MARY-X,an IBC cell line and primary xenograft,by virtue of increased aldehyde dehydrogenase (ALDH) activity as assessed by the ALDEFLUOR assay. Invasion and metastasis of CSC populations were assessed by in vitro and mouse xenograft assays. Expression of ALDH1 was determined on a retrospective series of 109 IBC patients and this was correlated with histoclinical data. All statistical tests were two sided. Log-rank tests using Kaplan-Meier analysis were used to determine the correlation of ALDH1 expression with development of metastasis and patient outcome. RESULTS: Both in vitro and xenograft assays showed that invasion and metastasis in IBC are mediated by a cellular component that displays ALDH activity. Furthermore,expression of ALDH1 in IBC was an independent predictive factor for early metastasis and decreased survival in this patient population. CONCLUSIONS: These results suggest that the metastatic,aggressive behavior of IBC may be mediated by a CSC component that displays ALDH enzymatic activity. ALDH1 expression represents the first independent prognostic marker to predict metastasis and poor patient outcome in IBC. The results illustrate how stem cell research can translate into clinical practice in the IBC field. View Publication

产品类型：

产品号#：

01700

01705

01701

01702

产品名：

ALDEFLUOR™ 试剂盒

ALDEFLUOR™ DEAB试剂

ALDEFLUOR™测定缓冲液

文献

Grudzien P et al. (OCT 2010) Anticancer research 30 10 3853--67

Inhibition of Notch signaling reduces the stem-like population of breast cancer cells and prevents mammosphere formation.

BACKGROUND: Cancer stem cells (CSCs) are believed to be responsible for breast cancer formation and recurrence; therefore,therapeutic strategies targeting CSCs must be developed. One approach may be targeting signaling pathways,like Notch,that are involved in stem cell self-renewal and survival. MATERIALS AND METHODS: Breast cancer stem-like cells derived from cell lines and patient samples were examined for Notch expression and activation. The effect of Notch inhibition on sphere formation,proliferation,and colony formation was determined. RESULTS: Breast cancer stem-like cells consistently expressed elevated Notch activation compared with bulk tumor cells. Blockade of Notch signaling using pharmacologic and genomic approaches prevented sphere formation,proliferation,and/or colony formation in soft agar. Interestingly,a gamma-secretase inhibitor,MRK003,induced apoptosis in these cells. CONCLUSION: Our findings support a crucial role for Notch signaling in maintenance of breast cancer stem-like cells,and suggest Notch inhibition may have clinical benefits in targeting CSCs. View Publication

产品类型：

产品号#：

01700

01705

产品名：

ALDEFLUOR™ 试剂盒

ALDEFLUOR™ DEAB试剂

1:23

视频

Tools for Breast Cancer Research

发布日期: 1/25/10

产品手册

Reliable Antibodies for Your Research

产品类型：

品牌：

EasySep

产品号#：

60100

60100.1

60100AD

60100AD.1

60102

60102FI

60104

60104AD

60104AD.1

60104AZ

60104AZ.1

60104BT

60104BT.1

60104BT.2

60104FI

60104FI.1

60104PE

60104PE.1

60106

60106.1

60106AZ

60106AZ.1

60106BT

60106BT.1

60106FI

60106FI.1

60106PE

60106PE.1

60107

60107.1

601

产品名：

抗β-微管蛋白III抗体，克隆AA10

抗β-微管蛋白III抗体，克隆AA10，Alexa Fluor® 488

抗β-微管蛋白III抗体，clone AA10，Alexa Fluor® 488

抗小鼠TCR Gamma/Delta抗体，clone GL3

抗小鼠TCR Gamma/Delta抗体，clone GL3，Alexa Fluor® 488

抗小鼠TCR Gamma/Delta抗体，clone GL3，APC

抗小鼠TCR Gamma/Delta抗体，clone GL3，Biotin

抗小鼠TCR Gamma/Delta抗体，clone GL3，FITC

抗小鼠TCR Gamma/Delta抗体，clone GL3，PE

抗人CD71（转铁蛋白受体）抗体，clone OKT9

抗人CD71（转铁蛋白受体）抗体，clone OKT9，APC

抗人CD71（转铁蛋白受体）抗体，clone OKT9，Biotin

抗人CD71（转铁蛋白受体）抗体，clone OKT9，FITC

抗人CD71（转铁蛋白受体）抗体，clone OKT9，PE

科学海报

HepatiCult™ for Human Hepatic Organoids: A Validated Culture System for Drug Toxicity Screening

产品类型：

产品号#：

产品名：

Patient-Derived Organoids for Drug Screening and Development

50:25

线上讲座

Patient-Derived Organoids for Drug Screening and Development

发布日期: 7/25/17

文献

Pino CJ et al. (FEB 2013) Nephrology,dialysis,transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 28 2 296--302

Cell-based approaches for the treatment of systemic inflammation.

Acute and chronic solid organ failures are costly disease processes with high mortality rates. Inflammation plays a central role in both acute and chronic organ failure,including heart,lung and kidney. In this regard,new therapies for these disorders have focused on inhibiting the mediators of inflammation,including cytokines and free radicals,with little or no success in clinical studies. Recent novel treatment strategies have been directed to cell-based rather than mediator-based approaches,designed to immunomodulate the deleterious effects of inflammation on organ function. One approach,cell therapy,replaces cells that were damaged in the acute or chronic disease process with stem/progenitor technology,to rebalance excessive inflammatory states. As an example of this approach,the use of an immunomodulatory role of renal epithelial progenitor cells to treat acute renal failure (ARF) and multiorgan failure arising from acute kidney injury is reviewed. A second therapeutic pathway,cell processing,does not incorporate stem/progenitor cells in the device,but rather biomimetic materials that remove and modulate the primary cellular components,which promote the worsening organ tissue injury associated with inflammation. The use of an immunomodulating leukocyte selective cytopheretic inhibitory device is also reviewed as an example of this cell processing approach. Both of these unconventional strategies have shown early clinical efficacy in pilot clinical trials and may transform the therapeutic approach to organ failure disorders. View Publication

产品类型：

产品号#：

07930

07931

07940

07955

07956

07959

07954

产品名：

CryoStor® CS10

文献

Murphy S et al. (APR 2010) Current protocols in stem cell biology Chapter 1 Unit 1E.6

Amnion epithelial cell isolation and characterization for clinical use.

Human amnion epithelial cells (hAECs) are a heterologous population positive for stem cell markers; they display multilineage differentiation potential,differentiating into cells of the endoderm (liver,lung epithelium),mesoderm (bone,fat),and ectoderm (neural cells). They have a low immunogenic profile and possess potent immunosuppressive properties. Hence,hAECs may be a valuable source of cells for cell therapy. This unit describes an efficient and effective method of hAEC isolation,culture,and cryopreservation that is animal product-free and in accordance with current guidelines on preparation of cells for clinical use. Cells isolated using this method were characterized after 5 passages by analysis of karyotype,cell cycle distribution,and changes in telomere length. The differentiation potential of hAECs isolated using this animal product-free method was demonstrated by differentiation into lineages of the three primary germ layers and expression of lineage-specific markers analyzed by PCR,immunocytochemistry,and histology. View Publication

产品类型：

产品号#：

07930

07931

07940

07955

07956

07959

07954

产品名：

CryoStor® CS10

Using Human Pluripotent Stem Cell-Derived Neural Organoids for Disease Modeling

31:49

线上讲座

Using Human Pluripotent Stem Cell-Derived Neural Organoids for Disease Modeling

发布日期: 8/1/24

科学海报

Human Intestinal Organoid Culture System for Drug-Induced Gastrointestinal Toxicity Screening

产品类型：

Conference：

SOT 2023

产品号#：

产品名：

搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

Aldehyde dehydrogenase 1-positive cancer stem cells mediate metastasis and poor clinical outcome in inflammatory breast cancer.

Inhibition of Notch signaling reduces the stem-like population of breast cancer cells and prevents mammosphere formation.

Cell-based approaches for the treatment of systemic inflammation.

Amnion epithelial cell isolation and characterization for clinical use.

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