搜索结果: 'easysep direct'

科学海报
Isolation of Highly Purified Mouse CD4+CD25+Foxp3+ Regulatory T Cells in Less

产品类型：

Conference：

AAI 2014

产品号#：

18782

18782RF

产品名：

EasySep™小鼠CD25调节性T细胞正选试剂盒

RoboSep™ 小鼠CD25调节性T细胞正选试剂盒
科学海报
Isolation of Eosinophils Without Ficoll or RBC Lysis

产品类型：

Conference：

AAI 2007

产品号#：

19256

19256RF

21000

20155

20119

产品名：

RoboSep™- S

RoboSep™分选管套装（9个塑料管）

RoboSep™ 吸头组件抛光剂
产品手册
Reliable Antibodies for Your Research

产品类型：

品牌：

EasySep

产品号#：

60100

60100.1

60100AD

60100AD.1

60102

60102FI

60104

60104AD

60104AD.1

60104AZ

60104AZ.1

60104BT

60104BT.1

60104BT.2

60104FI

60104FI.1

60104PE

60104PE.1

60106

60106.1

60106AZ

60106AZ.1

60106BT

60106BT.1

60106FI

60106FI.1

60106PE

60106PE.1

60107

60107.1

601

产品名：

抗β-微管蛋白III抗体，克隆AA10

抗β-微管蛋白III抗体，克隆AA10

抗β-微管蛋白III抗体，克隆AA10，Alexa Fluor® 488

抗β-微管蛋白III抗体，clone AA10，Alexa Fluor® 488

抗小鼠TCR Gamma/Delta抗体，clone GL3

抗小鼠TCR Gamma/Delta抗体，clone GL3，Alexa Fluor® 488

抗小鼠TCR Gamma/Delta抗体，clone GL3，APC

抗小鼠TCR Gamma/Delta抗体，clone GL3，APC

抗小鼠TCR Gamma/Delta抗体，clone GL3，Biotin

抗小鼠TCR Gamma/Delta抗体，clone GL3，Biotin

抗小鼠TCR Gamma/Delta抗体，clone GL3，FITC

抗小鼠TCR Gamma/Delta抗体，clone GL3，PE

抗小鼠TCR Gamma/Delta抗体，clone GL3，PE

抗人CD71（转铁蛋白受体）抗体，clone OKT9

抗人CD71（转铁蛋白受体）抗体，clone OKT9

抗人CD71（转铁蛋白受体）抗体，clone OKT9，APC

抗人CD71（转铁蛋白受体）抗体，clone OKT9，APC

抗人CD71（转铁蛋白受体）抗体，clone OKT9，Biotin

抗人CD71（转铁蛋白受体）抗体，clone OKT9，FITC

抗人CD71（转铁蛋白受体）抗体，clone OKT9，FITC

抗人CD71（转铁蛋白受体）抗体，clone OKT9，PE

抗人CD71（转铁蛋白受体）抗体，clone OKT9，PE
产品手册
使用RoboSep™ 进行全自动细胞分选

产品类型：

品牌：

EasySep

产品号#：

23000

23101

23102

23103

20104

20124

21000

20119

20155

19641

19641RF

19642

19642RF

19643

19643RF

19644

19644RF

20164

18644

18644RF

产品名：

RoboSep™-16

RoboSep™-16导电滤芯吸头, 无菌

RoboSep™-16导电滤芯吸头

RoboSep™-16垃圾袋, 每包100个

RoboSep™ 缓冲液

RoboSep™ 缓冲液（5X浓缩液）

RoboSep™- S

RoboSep™ 吸头组件抛光剂

RoboSep™分选管套装（9个塑料管）

EasySep™大鼠T细胞分选试剂盒

RoboSep™ 大鼠T细胞分选试剂盒

EasySep™大鼠CD4+ T细胞分选试剂盒

RoboSep™ 大鼠CD4+ T细胞分选试剂盒

EasySep™大鼠CD8+ T细胞分选试剂盒

RoboSep™ 大鼠CD8+ T细胞分选试剂盒

EasySep™大鼠B细胞分选试剂盒

RoboSep™ 大鼠B细胞分选试剂盒

RoboSep™ 缓冲液 2

EasySep™大鼠IgM去除试剂盒

RoboSep™ 大鼠IgM去除试剂盒
挂图
血液相关来源中人细胞类型的比例 List of the frequencies of over 35 cell types in normal human blood-related sources.
技术窍门
流式细胞术的设门方法及注意事项
产品手册
细胞分选相关工具

产品类型：

品牌：

EasySep

产品号#：

07932

07933

07953

07949

07801

07811

07851

07861

10970

10990

10981

18062

18062RF

18063

17851

17851RF

17852

17852RF

17853

17853RF

17854

17854RF

17858

17858RF

17951

17951RF

17952

17952RF

17953

17953RF

18051

18051RF

18052

18052RF

18053

18053RF

18054

18054RF

1

产品名：

CryoStor®CS2

CryoStor®CS5

CryoStor®CS5

CryoStor®CS5

Lymphoprep™

Lymphoprep™

ImmunoCult™ 人CD3/CD28/CD2 T细胞激活剂

ImmunoCult™ 人CD3/CD28/CD2 T细胞激活剂

ImmunoCult™ XF 人T细胞扩增培养基，500 mL

EasySep™人CD4+CD127low CD25+调节性T细胞分选试剂盒

EasySep™人CD3正选试剂盒II

RoboSep™ 人CD3正选试剂盒II

EasySep™人CD4正选试剂盒II

RoboSep™ 人CD4正选试剂盒II

EasySep™人CD8正选试剂盒 II

RoboSep™ 人CD8正选试剂盒 II

EasySep™人CD19正选试剂盒II

RoboSep™ 人CD19正选试剂盒II

EasySep™人CD14正选试剂盒II

RoboSep™ 人CD14正选试剂盒II

EasySep™人T细胞分选试剂盒

RoboSep™ 人T细胞分选试剂盒

EasySep™人CD4+ T细胞分选试剂盒

RoboSep™ 人CD4+ T细胞分选试剂盒

EasySep™人CD8+ T细胞分选试剂盒

RoboSep™ 人CD8+ T细胞分选试剂盒
挂图
Innate Lymphoid Cells Overview of innate lymphoid cells (ILCs) development, classification, plasticity and functional diversity
科学海报
Magnetic Enrichment of Mouse ILC2s from the Lung

产品类型：

产品号#：

19842

产品名：

EasySep™小鼠ILC2富集试剂盒
科学海报
Pre-enrichment of Human ILCs Improves Sorting Efficiency While Maintaining Functionality

产品类型：

Conference：

EMBO: ILCs 2016

产品号#：

17975

17975RF

产品名：

EasySep™人Pan-ILC富集试剂盒

RoboSep™ 人Pan-ILC富集试剂盒
Vu F et al. (FEB 2008) Journal of immunology (Baltimore,Md. : 1950) 180 4 2284--93

ICOS, CD40, and lymphotoxin beta receptors signal sequentially and interdependently to initiate a germinal center reaction.

Germinal center (GC) responses to T-dependent Ags require effective collaboration between Th cells,activated B cells,and follicular dendritic cells within a highly organized microenvironment. Studies using gene-targeted mice have highlighted nonredundant molecules that are key for initiating and maintaining the GC niche,including the molecules of the ICOS,CD40,and lymphotoxin (LT) pathways. Signaling through ICOS has multiple consequences,including cytokine production,expression of CD40L on Th cells,and differentiation into CXCR5(+) follicular Th cells,all of which are important in the GC reaction. We have therefore taken advantage of ICOS(-/-) mice to dissect which downstream elements are required to initiate the formation of GC. In the context of a T-dependent immune response,we found that GC B cells from ICOS(-/-) mice express lower levels of LTalphabeta compared with wild-type GC B cells in vivo,and stimulation of ICOS on T cells induces LTalphabeta on B cells in vitro. Administration of agonistic anti-LTbeta receptor Ab was unable to restore the GC response in ICOS(-/-) mice,suggesting that additional input from another pathway is required for optimal GC generation. In contrast,treatment with agonistic anti-CD40 Ab in vivo recovered GC networks and restored LTalphabeta expression on GC B cells in ICOS(-/-) mice,and this effect was dependent on LTbeta receptor signaling. Collectively,these data demonstrate that ICOS activation is a prerequisite for the up-regulation of LTalphabeta on GC B cells in vivo and provide a model for cooperation between ICOS,CD40,and LT pathways in the context of the GC response. View Publication

产品类型：

产品号#：

21000

20119

20155

19752

19752RF

19754

19754RF

产品名：

RoboSep™- S

RoboSep™ 吸头组件抛光剂

RoboSep™分选管套装（9个塑料管）
Fogli M et al. (JUL 2008) PLoS pathogens 4 7 e1000101

Lysis of endogenously infected CD4+ T cell blasts by rIL-2 activated autologous natural killer cells from HIV-infected viremic individuals.

Understanding the cellular mechanisms that ensure an appropriate innate immune response against viral pathogens is an important challenge of biomedical research. In vitro studies have shown that natural killer (NK) cells purified from healthy donors can kill heterologous cell lines or autologous CD4+ T cell blasts exogenously infected with several strains of HIV-1. However,it is not known whether the deleterious effects of high HIV-1 viremia interferes with the NK cell-mediated cytolysis of autologous,endogenously HIV-1-infected CD4+ T cells. Here,we stimulate primary CD4+ T cells,purified ex vivo from HIV-1-infected viremic patients,with PHA and rIL2 (with or without rIL-7). This experimental procedure allows for the significant expansion and isolation of endogenously infected CD4+ T cell blasts detected by intracellular staining of p24 HIV-1 core antigen. We show that,subsequent to the selective down-modulation of MHC class-I (MHC-I) molecules,HIV-1-infected p24(pos) blasts become partially susceptible to lysis by rIL-2-activated NK cells,while uninfected p24(neg) blasts are spared from killing. This NK cell-mediated killing occurs mainly through the NKG2D activation pathway. However,the degree of NK cell cytolytic activity against autologous,endogenously HIV-1-infected CD4+ T cell blasts that down-modulate HLA-A and -B alleles and against heterologous MHC-I(neg) cell lines is particularly low. This phenomenon is associated with the defective surface expression and engagement of natural cytotoxicity receptors (NCRs) and with the high frequency of the anergic CD56(neg)/CD16(pos) subsets of highly dysfunctional NK cells from HIV-1-infected viremic patients. Collectively,our data demonstrate that the chronic viral replication of HIV-1 in infected individuals results in several phenotypic and functional aberrancies that interfere with the NK cell-mediated killing of autologous p24(pos) blasts derived from primary T cells. View Publication

产品类型：

产品号#：

19052

19052RF

19055

19055RF

产品名：

EasySep™人CD4+ T细胞富集试剂盒

RoboSep™ 人CD4+ T细胞富集试剂盒含滤芯吸头

EasySep™人NK细胞富集试剂盒

RoboSep™ 人NK细胞富集试剂盒含滤芯吸头