搜索结果: 'methocult media formulations for human hematopoietic cells serum containing'

健康与疾病状态的人造血集落 健康与疾病状态的人造血集落图谱
RosetteSep™人造血祖细胞富集抗体混合物 免疫密度负选试剂混合物
人iPSC衍生的间充质祖细胞 人诱导多能干细胞系SCTi003-A分化的冻存的间充质祖细胞
MegaCult™-C cfu染色试剂盒 人CFU-Mk染色试剂盒
新品
人iPSC衍生的小胶质细胞 从人诱导多能干细胞系SCTi003-A分化而来的冻存小胶质细胞
冻存的ACF培养的人外周血来源的成熟树突状细胞 冻存的人原代细胞
NeuroCult™成年中枢神经系统（CNS）组织酶解试剂盒（小鼠和大鼠） 成年小鼠和大鼠CNS组织酶解试剂盒
冻存的ACF培养的人外周血来源的未成熟树突状细胞 冻存的人原代细胞
产品手册
MethoCult™ Media for Performing Hematopoietic Colony-Forming Unit (CFU) Assays

产品类型：

品牌：

MethoCult

产品号#：

03134

03231

03234

03334

03434

03436

03444

03534

03630

04064

04100

04230

04236

04431

04434

04436

04444

04464

04531

04535

04536

04545

04564

07700

07800

07850

27100

27150

28110

28120

28230

28240

38068

产品名：

MethoCult™M3134

MethoCult™M3231

MethoCult™M3234

MethoCult™M3334

MethoCult™GF M3434

MethoCult™SF M3436

MethoCult™GF M3434

MethoCult™GF M3534

MethoCult™M3630

MethoCult™ H4034 Optimum启动试剂盒套装

MethoCult™ H4100

MethoCult™H4230

MethoCult™SF H4236

MethoCult™H4431

MethoCult™H4434经典

MethoCult™ SF H4436

MethoCult™H4434经典

MethoCult™ H4434 Classic启动试剂盒套装

MethoCult™H4531

MethoCult™H4535富集无EPO

MethoCult™ SF H4536

MethoCult™ H4535 Enriched，不含EPO

入门套件MethoCult™H4534经典无EPO

含 2% FBS的Iscove's MDM

氯化铵溶液

氯化铵溶液

35 mm培养皿

35 mm培养皿

16号钝针

16号钝针

3 mL注射器

3 mL注射器

Corning® 60 mm 带网格培养皿
人IL-17A酶联免疫吸附测定试剂盒 用于人白细胞介素17A的检测和测定
Lu S-J et al. (JUL 2013) Regenerative medicine 8 4 413--424

3D microcarrier system for efficient differentiation of human pluripotent stem cells into hematopoietic cells without feeders and serum [corrected].

BACKGROUND Human embryonic stem cells (hESCs) have been derived and maintained on mouse embryonic fibroblast feeders to keep their undifferentiated status. To realize their clinical potential,a feeder-free and scalable system for large scale production of hESCs and their differentiated derivatives is required. MATERIALS & METHODS hESCs were cultured and passaged on serum/feeder-free 3D microcarriers for five passages. For embryoid body (EB) formation and hemangioblast differentiation,the medium for 3D microcarriers was directly switched to EB medium. RESULTS hESCs on 3D microcarriers maintained pluripotency and formed EBs,which were ten-times more efficient than hESCs cultured under 2D feeder-free conditions (0.11 ± 0.03 EB cells/hESC input 2D vs 1.19 ± 0.32 EB cells/hESC input 3D). After replating,EB cells from 3D culture readily developed into hemangioblasts with the potential to differentiate into hematopoietic and endothelial cells. Furthermore,this 3D system can also be adapted to human induced pluripotent stem cells,which generate functional hemangioblasts with high efficiency. CONCLUSION This 3D serum- and stromal-free microcarrier system is important for future clinical applications,with the potential of developing to a GMP-compatible scalable system. View Publication

产品类型：

产品号#：

04436

85850

85857

产品名：

MethoCult™ SF H4436

mTeSR™1

mTeSR™1
MesenCult™ 成骨细胞分化试剂盒 (人) 用于体外诱导人MSC分化为成骨细胞