EasySep™小鼠TIL（CD45）正选试剂盒
肝类器官培养
生成肝类器官的完整、明确的培养基
为什么使用 HepatiCult™ ？
- 体外系统，可在一周内生成类器官。
- 无需建立损伤模型、手工挑选导管或进行细胞分选
- 无血清且成分明确的培养基配方
- 类器官既可从导管片段或单细胞起始培养，也可在基质凝固液滴或悬浮体系中生长
肝类器官培养产品
品牌的历史
尽管肝脏在体内具有显著的再生能力，但肝组织的体外长期培养直至近年才取得突破。在2013年, Meritxell Huch博士等人 首次报道了可稳定维持肝组织类器官的培养体系。2014年，Hans Clevers博士和 Hubrecht Organoid Technology 基金会与 STEMCELL Technologies 达成协议 ，制造和销售类器官的细胞培养基。2018年初推出的HepatiCult™类器官生长培养基（小鼠），为研究人员提供了建立和维持肝祖类器官的便捷方案。
Key Applications of Hepatic Organoids
Adult-Tissue Derived Liver Organoids
Broutier L, et al. (2016) Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation. Nat Protoc. 11(9): 1724-43.
Huch M, et al. (2015) Long-term culture of genome-stable bipotent stem cells from adult human liver. Cell. 160(1-2): 299-312.
Huch M, et al. (2013) In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration. Nature. 494(7436): 247-50.
Kuijk EW, et al. (2016) Generation and characterization of rat liver stem cell lines and their engraftment in a rat model of liver failure. Sci Rep. 6: 22154.
Nantasanti S, et al. (2015) Disease modeling and gene therapy of copper storage disease in canine hepatic organoids. Stem Cell Reports. 5(5): 895-907.
Disease Modeling and Cancer Research
Lampis A, et al. (2017) MIR21 drives resistance to Heat Shock Protein 90 inhibition in cholangiocarcinoma. Gastroenterology. S0016-5085(17): 36331-X.
Broutier L, et al. (2017) Human primary liver cancer-derived organoid cultures for disease modelling and drug screening. Nat Med. 23(12): 1424-1435.
Sampaziotis F, et al. (2015) Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation. Nat Biotechnol. 33(8): 845-852.
Hindley CJ, et al. (2016) Organoids from adult liver and pancreas: Stem cell biology and biomedical utility. Dev Biol. 420(2): 251-261.
Hepatic Cell Biology
Dorrell C, et al. (2014) The organoid-initiating cells in mouse pancreas and liver are phenotypically and functionally similar. Stem Cell Res. 13(2): 275-83.
Tarlow BD, et al. (2014) Clonal tracing of Sox9+ liver progenitors in mouse oval cell injury. Hepatology. 60(1): 278-89.
Yimlamai D, et al. (2014) Hippo pathway activity influences liver cell fate. Cell. 157(6): 1324-38.
Kruitwagen HS, et al. (2018) DYRK1A Is a Regulator of S-Phase Entry in Hepatic Progenitor Cells. Stem Cells Dev.
hPSC-Derived Liver Organoids
Guye P, et al. (2016) Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Nat Commun. 7: 10243.
Takebe T, et al. (2017) Massive and reproducible production of liver buds entirely from human pluripotent stem cells. Cell Rep. 21(10):2661-2670.
Takebe T, et al. (2014) Generation of a vascularized and functional human liver from an iPSC-derived organ bud transplant. Nat Protoc. 9(2): 396-409.
Organoids in Multi-Lineage Model Systems
Kasuya J, et al. (2015) Reconstruction of hepatic stellate cell-incorporated liver capillary structures in small hepatocyte tri-culture using microporous membranes. J Tissue Eng Regen Med. 9(3): 247-56.
Takebe T, et al. (2012) Self-Organization of human hepatic organoid by recapitulating organogenesis in vitro. Transplant Proc. 44(4): 1018-20.
Skardal A, et al. (2015) Liver-tumor hybrid organoids for modeling tumor growth and drug response in vitro. Ann Biomed Eng. 43(10): 2361-73.
