搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

研究综述

Neural Stem Cells: Identification, Function, Culture, and Isolation

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细胞类型：

神经干祖细胞

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05700

05701

05702

05703

05704

05707

05715

05740

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05750

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00341

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NeuroCult™ 基础培养基（小鼠和大鼠）

NeuroCult™ 扩增添加物（小鼠和大鼠）

NeuroCult™扩增试剂盒（小鼠和大鼠）

NeuroCult™ 分化添加物（小鼠和大鼠）

NeuroCult™ 分化试剂盒（小鼠和大鼠）

NeuroCult™化学解离试剂盒（小鼠）

NeuroCult™成年中枢神经系统（CNS）组织酶解试剂盒（小鼠和大鼠）

NeuroCult™ NS-A 基础培养基（人）

NeuroCult™ NS-A 扩增试剂盒（人）

NeuroCult™ NS-A 分化试剂盒（人）

用于小鼠和大鼠神经干细胞和祖细胞分化培养的试剂盒

发布日期: 04/01/2015

技术手册

Maintenance of Human Pluripotent Stem Cells in TeSR™2

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05860

05880

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Miyoshi N et al. (JAN 2010) Proceedings of the National Academy of Sciences of the United States of America 107 1 40--5

Defined factors induce reprogramming of gastrointestinal cancer cells.

Although cancer is a disease with genetic and epigenetic origins,the possible effects of reprogramming by defined factors remain to be fully understood. We studied the effects of the induction or inhibition of cancer-related genes and immature status-related genes whose alterations have been reported in gastrointestinal cancer cells. Retroviral-mediated introduction of induced pluripotent stem (iPS) cell genes was necessary for inducing the expression of immature status-related proteins,including Nanog,Ssea4,Tra-1-60,and Tra-1-80 in esophageal,stomach,colorectal,liver,pancreatic,and cholangiocellular cancer cells. Induced cells,but not parental cells,possessed the potential to express morphological patterns of ectoderm,mesoderm,and endoderm,which was supported by epigenetic studies,indicating methylation of DNA strands and the histone H3 protein at lysine 4 in promoter regions of pluripotency-associated genes such as NANOG. In in vitro analysis induced cells showed slow proliferation and were sensitized to differentiation-inducing treatment,and in vivo tumorigenesis was reduced in NOD/SCID mice. This study demonstrated that pluripotency was manifested in induced cells,and that the induced pluripotent cancer (iPC) cells were distinct from natural cancer cells with regard to their sensitivity to differentiation-inducing treatment. Retroviral-mediated introduction of iPC cells confers higher sensitivity to chemotherapeutic agents and differentiation-inducing treatment. View Publication

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05850

05857

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mTeSR™1

Ishizawa K et al. (SEP 2010) Cell stem cell 7 3 279--82

Tumor-initiating cells are rare in many human tumors.

Tumor-initiating cells (TICs) are defined by their ability to form tumors after xenotransplantation in immunodeficient mice and appear to be relatively rare in most human cancers. Recent data in melanoma indicate that the frequency of TICs increases dramatically via more permissive xenotransplantation conditions,raising the possibility that the true frequency of TICs has been greatly underestimated in most human tumors. We compared the growth of human pancreatic,non-small cell lung,and head and neck carcinomas in NOD/SCID and NSG mice. Although TIC frequency was detected up to 10-fold higher in NSG mice,it remained low (textless1 in 2500 cells) in all cases. Moreover,aldehyde dehydrogenase-positive (ALDH(+)) and CD44(+)CD24(+) cells,phenotypically distinct cells enriched in TICs,were equally tumorigenic in NOD/SCID and NSG mice. Our findings demonstrate that TICs are rare in these cancers and that the identification of TICs and their frequency in other human malignancies should be validated via primary tumors and highly permissive xenotransplantation conditions. View Publication

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01700

01705

01701

01702

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ALDEFLUOR™ 试剂盒

ALDEFLUOR™ DEAB试剂, 1.5 mM, 1 mL

ALDEFLUOR™检测缓冲液

Boheler KR et al. (AUG 2002) Circulation research 91 3 189--201

Differentiation of pluripotent embryonic stem cells into cardiomyocytes.

Embryonic stem (ES) cells have been established as permanent lines of undifferentiated pluripotent cells from early mouse embryos. ES cells provide a unique system for the genetic manipulation and the creation of knockout strains of mice through gene targeting. By cultivation in vitro as 3D aggregates called embryoid bodies,ES cells can differentiate into derivatives of all 3 primary germ layers,including cardiomyocytes. Protocols for the in vitro differentiation of ES cells into cardiomyocytes representing all specialized cell types of the heart,such as atrial-like,ventricular-like,sinus nodal-like,and Purkinje-like cells,have been established. During differentiation,cardiac-specific genes as well as proteins,receptors,and ion channels are expressed in a developmental continuum,which closely recapitulates the developmental pattern of early cardiogenesis. Exploitation of ES cell-derived cardiomyocytes has facilitated the analysis of early cardiac development and has permitted in vitro gain-of-function" or "loss-of-function" genetic studies. Recently� View Publication

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Ben-David U and Benvenisty N (MAR 2014) Nature protocols 9 3 729--740

Chemical ablation of tumor-initiating human pluripotent stem cells.

The tumorigenicity of human pluripotent stem cells (hPSCs) is widely acknowledged as a major obstacle that withholds their application in regenerative medicine. This protocol describes two efficient and robust ways to chemically eliminate the tumor-initiating hPSCs from monolayer culture. The protocol details how to maintain and differentiate hPSCs,how to apply chemical inhibitors to cultures of hPSCs and their differentiated progeny,and how to assess the purity of the resultant cell cultures using in vitro and in vivo assays. It also describes how to rescue the cytotoxic effect. The elimination and the rescue assay can be completed within 3-5 d,the in vitro assessment requires another day,and the in vivo assessment requires up to 12 additional weeks. View Publication

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05850

05857

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mTeSR™1

Ichida JK et al. (AUG 2014) Nature chemical biology 10 8 632--9

Notch inhibition allows oncogene-independent generation of iPS cells.

The reprogramming of somatic cells to pluripotency using defined transcription factors holds great promise for biomedicine. However,human reprogramming remains inefficient and relies either on the use of the potentially dangerous oncogenes KLF4 and CMYC or the genetic inhibition of the tumor suppressor gene p53. We hypothesized that inhibition of signal transduction pathways that promote differentiation of the target somatic cells during development might relieve the requirement for non-core pluripotency factors during induced pluripotent stem cell (iPSC) reprogramming. Here,we show that inhibition of Notch greatly improves the efficiency of iPSC generation from mouse and human keratinocytes by suppressing p21 in a p53-independent manner and thereby enriching for undifferentiated cells capable of long-term self-renewal. Pharmacological inhibition of Notch enabled routine production of human iPSCs without KLF4 and CMYC while leaving p53 activity intact. Thus,restricting the development of somatic cells by altering intercellular communication enables the production of safer human iPSCs. View Publication

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05850

05857

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73092

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DBZ

mTeSR™1

Kurian L et al. (JAN 2013) Nature methods 10 1 77--83

Conversion of human fibroblasts to angioblast-like progenitor cells.

Lineage conversion of one somatic cell type to another is an attractive approach for generating specific human cell types. Lineage conversion can be direct,in the absence of proliferation and multipotent progenitor generation,or indirect,by the generation of expandable multipotent progenitor states. We report the development of a reprogramming methodology in which cells transition through a plastic intermediate state,induced by brief exposure to reprogramming factors,followed by differentiation. We use this approach to convert human fibroblasts to mesodermal progenitor cells,including by non-integrative approaches. These progenitor cells demonstrated bipotent differentiation potential and could generate endothelial and smooth muscle lineages. Differentiated endothelial cells exhibited neo-angiogenesis and anastomosis in vivo. This methodology for indirect lineage conversion to angioblast-like cells adds to the armamentarium of reprogramming approaches aimed at the study and treatment of ischemic pathologies. View Publication

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05850

05857

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05875

85850

85857

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产品名：

mTeSR™1

Ward E et al. (MAY 2017) Stem cells and development

Feeder-Free Derivation of Naïve Human Pluripotent Stem Cells.

Human pluripotent stem cells (HPSCs) cultured in conditions that maintain pluripotency via FGF and TGFβ signaling have been described as being in a primed state. These cells have been shown to exhibit characteristics more closely related to mouse epiblast-derived stem cells than to so called naïve mouse PSCs said to possess a more ground state pluripotency that mimics the early mouse embryo inner cell mass. Initial attempts to create culture conditions favorable for generation of naïve HPSCs from primed HPSCs has required the use of mouse embryonic fibroblasts as a feeder layer to support this transition. A protocol for the routine derivation and maintenance of naïve HPSCs in completely defined conditions is highly desirable for stem cell researchers to enhance the study and clinical translation of naïve HPSCs. Here we describe a standard protocol for transitioning primed HPSCs to a naïve state using commercial RSet media and xeno-free recombinant vitronectin. View Publication

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05230

05970

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STEMdiff™ 三胚层分化试剂盒

Consistently Differentiating Astrocytes from Human Pluripotent Stem Cells

42:05

线上讲座

Consistently Differentiating Astrocytes from Human Pluripotent Stem Cells

发布日期: 12/09/2024

(Feb 2024) iScience 27 3

A1-reprogrammed mesenchymal stromal cells prime potent antitumoral responses

SummaryMesenchymal stromal cells (MSCs) have been modified via genetic or pharmacological engineering into potent antigen-presenting cells-like capable of priming responding CD8 T cells. In this study,our screening of a variant library of Accum molecule revealed a molecule (A1) capable of eliciting antigen cross-presentation properties in MSCs. A1-reprogrammed MSCs (ARM) exhibited improved soluble antigen uptake and processing. Our comprehensive analysis,encompassing cross-presentation assays and molecular profiling,among other cellular investigations,elucidated A1’s impact on endosomal escape,reactive oxygen species production,and cytokine secretion. By evaluating ARM-based cellular vaccine in mouse models of lymphoma and melanoma,we observe significant therapeutic potency,particularly in allogeneic setting and in combination with anti-PD-1 immune checkpoint inhibitor. Overall,this study introduces a strong target for developing an antigen-adaptable vaccination platform,capable of synergizing with immune checkpoint blockers to trigger tumor regression,supporting further investigation of ARMs as an effective and versatile anti-cancer vaccine. Graphical abstract Highlights•Treatment with A1/antigen mix reprograms MSCs into antigen-presenting cells•The antigen cross-presenting ability of ARM cells require ROS and UPR•ARMs synergize with immune-checkpoint inhibitors in priming potent antitumoral activity Classification Description: Immunology; Pharmaceutical engineering; Cancer View Publication

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产品号#：

18953

18953RF

产品名：

EasySep™小鼠CD8a正选试剂盒II

RoboSep™ 小鼠CD8a正选试剂盒II

挂图

Derivation and Applications of Human Pluripotent Stem Cells Overview of the derivation of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs)

发布日期: 11/26/2020

搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

Defined factors induce reprogramming of gastrointestinal cancer cells.

Tumor-initiating cells are rare in many human tumors.

Differentiation of pluripotent embryonic stem cells into cardiomyocytes.

Chemical ablation of tumor-initiating human pluripotent stem cells.

Notch inhibition allows oncogene-independent generation of iPS cells.

Conversion of human fibroblasts to angioblast-like progenitor cells.

Feeder-Free Derivation of Naïve Human Pluripotent Stem Cells.

A1-reprogrammed mesenchymal stromal cells prime potent antitumoral responses

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