搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

NeuroCult™ 扩增添加物（小鼠和大鼠） 用于扩增小鼠和大鼠神经干细胞和祖细胞的添加物
文献

Al-Ali H et al. (MAY 2013) ACS chemical biology 8 5 1027--36

Applications for ROCK kinase inhibition.

ROCK kinases,which play central roles in the organization of the actin cytoskeleton,are tantalizing targets for the treatment of human diseases. Deletion of ROCK I in mice revealed a role in the pathophysiological responses to high blood pressure,and validated ROCK inhibition for the treatment of specific types of cardiovascular disease. To date,the only ROCK inhibitor employed clinically in humans is fasudil,which has been used safely in Japan since 1995 for the treatment of cerebral vasospasm. Clinical trials,mostly focusing on the cardiovascular system,have uncovered beneficial effects of fasudil for additional indications. Intriguing recent findings also suggest significant potential for ROCK inhibitors in the production and implantation of stem cells for disease therapies. View Publication

产品类型：

产品号#：

73802

73804

产品名：

Rho激酶抑制剂IV (Dihydrochloride)

Rho激酶抑制剂IV (Dihydrochloride)
产品手册
qPCR Arrays for Cell Characterization

产品类型：

Cell Dyes and Detection Assay Kits

品牌：

MesenCult，STEMdiff

产品号#：

05110

05220

05221

05401

05402

05411

05412

05455

05916

05946

05230

05120

03950

05240

85850

85857

05270

05275

05439

05990

07515

07516

07517

07521

07541

05876

产品名：

STEMdiff™权威内胚层检测试剂盒

STEMdiff™ 中胚层诱导培养基

STEMdiff™ 中胚层诱导培养基

MesenCult™ MSC 基础培养基（人）

MesenCult™ MSC 刺激补充剂（人）

MesenCult™ 增殖试剂盒（人）

MesenCult™ 脂肪分化试剂盒 (人)

MesenCult™-ACF软骨细胞分化试剂盒

TeSR™-E5

TeSR™-E6

STEMdiff™ 三胚层分化试剂盒

STEMdiff™胰腺祖细胞试剂盒

mTeSR™3 d

STEMdiff™ 间充质祖细胞试剂盒

mTeSR™1

mTeSR™1

STEMdiff™ APEL™2 培养基

STEMdiff™ APEL™2 培养基

MesenCult™-hPL 培养基试剂盒

用于hESC/hiPSC维持培养的TeSR™-E8™

人多能干细胞三谱系分化qPCR阵列

qPCR预混试剂盒

qPCR预混试剂盒

Naïve态人多能干细胞 qPCR 阵列

人间充质干细胞 qPCR 阵列分析

不含酚红的mTeSR™1
文献

Barbaric I et al. (JUL 2014) Stem Cell Reports 3 1 142--155

Time-lapse analysis of human embryonic stem cells reveals multiple bottlenecks restricting colony formation and their relief upon culture adaptation

Using time-lapse imaging,we have identified a series of bottlenecks that restrict growth of early-passage human embryonic stem cells (hESCs) and that are relieved by karyotypically abnormal variants that are selected by prolonged culture. Only a minority of karyotypically normal cells divided after plating,and these were mainly cells in the later stages of cell cycle at the time of plating. Furthermore,the daughter cells showed a continued pattern of cell death after division,so that few formed long-term proliferating colonies. These colony-forming cells showed distinct patterns of cell movement. Increasing cell density enhanced cell movement facilitating cell:cell contact,which resulted in increased proportion of dividing cells and improved survival postplating of normal hESCs. In contrast,most of the karyotypically abnormal cells reentered the cell cycle on plating and gave rise to healthy progeny,without the need for cell:cell contacts and independent of their motility patterns. ?? 2014 The Authors. View Publication

产品类型：

产品号#：

85850

85857

产品名：

mTeSR™1

mTeSR™1
Event
Association for Molecular Pathology (AMP)

产品类型：

产品号#：

产品名：
产品手册
Reliable Antibodies for Your Research

产品类型：

Antibodies

品牌：

EasySep，RoboSep，RosetteSep

产品号#：

60100

60100.1

60100AD

60100AD.1

60104

60104AD.1

60104AZ

60104AZ.1

60104BT

60104BT.1

60104FI.1

60104PE

60104PE.1

60106

60106.1

60106AZ

60106AZ.1

60106BT

60106FI

60106FI.1

60106PE

60106PE.1

产品名：

抗β-微管蛋白III抗体，克隆AA10

抗β-微管蛋白III抗体，克隆AA10

抗β-微管蛋白III抗体，克隆AA10，Alexa Fluor® 488

抗β-微管蛋白III抗体，克隆AA10，Alexa Fluor® 488

抗小鼠TCR Gamma/Delta 抗体，克隆GL3

抗小鼠TCR Gamma/Delta 抗体，克隆GL3，Alexa Fluor® 488

抗小鼠TCR Gamma/Delta 抗体，克隆GL3，APC

抗小鼠TCR Gamma/Delta 抗体，克隆GL3，APC

抗小鼠TCR Gamma/Delta 抗体，克隆GL3，Biotin

抗小鼠TCR Gamma/Delta 抗体，克隆GL3，Biotin

抗小鼠TCR Gamma/Delta 抗体，克隆GL3，FITC

抗小鼠TCR Gamma/Delta 抗体，克隆GL3，PE

抗小鼠TCR Gamma/Delta 抗体，克隆GL3，PE

抗人CD71（转铁蛋白受体）抗体，克隆OKT9

抗人CD71（转铁蛋白受体）抗体，克隆OKT9

抗人CD71（转铁蛋白受体）抗体，克隆OKT9，APC

抗人CD71（转铁蛋白受体）抗体，克隆OKT9，APC

抗人CD71（转铁蛋白受体）抗体，克隆OKT9，Biotin

抗人CD71（转铁蛋白受体）抗体，克隆OKT9，FITC

抗人CD71（转铁蛋白受体）抗体，克隆OKT9，FITC

抗人CD71（转铁蛋白受体）抗体，克隆OKT9，PE

抗人CD71（转铁蛋白受体）抗体，克隆OKT9，PE
产品手册
HLA Antibodies for Purity Assessment

产品类型：

Antibodies

品牌：

EasySep，RosetteSep

产品号#：

15276

15664HLA

15684HLA

60018

60018AD

60018AD.1

60018AZ

60018AZ.1

60018BT

60018FI

60018FI.1

60018PE

60018PE.1

60018PS

60018PS.1

60018PB

60018PB.1

60018PB.2

60022

60022AZ

60022AZ.1

60022FI

60022FI.1

60022PE

60022PE.1

60022PS

产品名：

RosetteSep™人脐带血祖细胞完全富集试剂盒

RosetteSep™ HLA粒细胞去除抗体混合物

RosetteSep™ HLA粒细胞去除抗体混合物

抗人CD45抗体，克隆HI30

抗人CD45抗体，克隆HI30，Alexa Fluor® 488

抗人CD45抗体，克隆HI30，Alexa Fluor® 488

抗人CD45抗体，克隆HI30，APC

抗人CD45抗体，克隆HI30，APC

抗人CD45抗体，克隆HI30，Biotin

抗人CD45抗体，克隆HI30，FITC

抗人CD45抗体，克隆HI30，FITC

抗人CD45抗体，克隆HI30，PE

抗人CD45抗体，克隆HI30，PE

抗人CD45抗体，克隆HI30，PerCP-Cy5.5

抗人CD45抗体，克隆HI30，PerCP-Cy5.5

抗人CD45抗体，克隆HI30，Pacific Blue™

抗人CD45抗体，克隆HI30，Pacific Blue™

抗人CD45抗体，克隆HI30，Pacific Blue™

抗人CD8a抗体，克隆RPA-T8

抗人CD8a抗体，克隆RPA-T8，APC

抗人CD8a抗体，克隆RPA-T8，APC

抗人CD8a抗体，克隆RPA-T8，FITC

抗人CD8a抗体，克隆RPA-T8，FITC

抗人CD8a抗体，克隆RPA-T8，PE

抗人CD8a抗体，克隆RPA-T8，PE

抗人CD8a抗体，克隆RPA-T8，PerCP-Cy5.5
技术窍门
Considerations for Flow Cytometry Gating
产品手册
Tools for Your Immunology Research

产品类型：

Cell Isolation Products

品牌：

EasySep，ImmunoCult，RoboSep，RosetteSep，SepMate

产品号#：

07932

07933

07953

07949

07811

07861

10970

10990

10981

18063

17851

17851RF

17852

17852RF

17853

17853RF

17854

17854RF

17858

17858RF

17951

17951RF

17952

17952RF

17953

17953RF

产品名：

CryoStor®CS2

CryoStor®CS5

CryoStor®CS5

CryoStor®CS5

Lymphoprep™

Lymphoprep™

ImmunoCult™ 人CD3/CD28/CD2 T细胞激活剂

ImmunoCult™ 人CD3/CD28/CD2 T细胞激活剂

ImmunoCult™ XF 人T细胞扩增培养基，500 mL

EasySep™人CD4+CD127low CD25+调节性T细胞分选试剂盒

EasySep™人CD3正选试剂盒II

RoboSep™ 人CD3正选试剂盒II

EasySep™人CD4正选试剂盒II

RoboSep™ 人CD4正选试剂盒II

EasySep™人CD8正选试剂盒 II

RoboSep™ 人CD8正选试剂盒 II

EasySep™人CD19正选试剂盒II

RoboSep™ 人CD19正选试剂盒II

EasySep™人CD14正选试剂盒II

RoboSep™ 人CD14正选试剂盒II

EasySep™人T细胞分选试剂盒

RoboSep™ 人T细胞分选试剂盒

EasySep™人CD4+ T细胞分选试剂盒

RoboSep™ 人CD4+ T细胞分选试剂盒

EasySep™人CD8+ T细胞分选试剂盒

RoboSep™ 人CD8+ T细胞分选试剂盒
24:55

线上讲座
Organoid-Based Assays for Disease Modeling

发布日期: 11/30/22
$G$\alpha$i2 and G$\alpha$i3 Differentially Regulate Arrest from Flow and Chemotaxis in Mouse Neutrophils.$

文献

Y. Kuwano et al. (MAY 2016) Journal of Immunology 196 9 3828--33

G$\alpha$i2 and G$\alpha$i3 Differentially Regulate Arrest from Flow and Chemotaxis in Mouse Neutrophils.

Leukocyte recruitment to inflammation sites progresses in a multistep cascade. Chemokines regulate multiple steps of the cascade,including arrest,transmigration,and chemotaxis. The most important chemokine receptor in mouse neutrophils is CXCR2,which couples through G$\alpha$i2- and G$\alpha$i3-containing heterotrimeric G proteins. Neutrophils arrest in response to CXCR2 stimulation. This is defective in G$\alpha$i2-deficient neutrophils. In this study,we show that G$\alpha$i3-deficient neutrophils showed reduced transmigration but normal arrest in mice. We also tested G$\alpha$i2- or G$\alpha$i3-deficient neutrophils in a CXCL1 gradient generated by a microfluidic device. G$\alpha$i3-,but not G$\alpha$i2-,deficient neutrophils showed significantly reduced migration and directionality. This was confirmed in a model of sterile inflammation in vivo. G$\alpha$i2-,but not G$\alpha$i3-,deficient neutrophils showed decreased Ca(2+) flux in response to CXCR2 stimulation. Conversely,G$\alpha$i3-,but not G$\alpha$i2-,deficient neutrophils exhibited reduced AKT phosphorylation upon CXCR2 stimulation. We conclude that G$\alpha$i2 controls arrest and G$\alpha$i3 controls transmigration and chemotaxis in response to chemokine stimulation of neutrophils. View Publication

产品类型：

产品号#：

19762

19762RF

产品名：

EasySep™小鼠中性粒细胞富集试剂盒

RoboSep™ 小鼠中性粒细胞富集试剂盒含滤芯吸头
0:52

视频
EasySep™ Mouse Cell Isolation Kits: How Much Time Will You Save? (Part 3)

发布日期: 5/8/13