搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

文献

Elliott DA et al. (DEC 2011) Nature methods 8 12 1037--1040

NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes.

NKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5 locus of human embryonic stem cells (hESCs); NKX2-5(eGFP/w) hESCs facilitate quantification of cardiac differentiation,purification of hESC-derived committed cardiac progenitor cells (hESC-CPCs) and cardiomyocytes (hESC-CMs) and the standardization of differentiation protocols. We used NKX2-5 eGFP(+) cells to identify VCAM1 and SIRPA as cell-surface markers expressed in cardiac lineages. View Publication

产品类型：

产品号#：

85850

85857

产品名：

mTeSR™1

mTeSR™1
科学海报
Fast and Easy Enrichment of Cell Subsets for HLA Analysis

产品类型：

Cell Isolation Products

Conference：

EFI 2012

产品号#：

85415

85420

85450

85460

产品名：

SepMate™-15 (IVD)

SepMate™-15 (IVD)

SepMate™-50 (IVD)

SepMate™-50 (IVD)
产品手册
ClonaCell™-HY Medium D for Robust Colony Formation During Semi-Solid Cloning

产品类型：

Cell Culture Media and Supplements

品牌：

ClonaCell

产品号#：

03800

03801

03802

03803

03804

03805

03806

03810

产品名：

ClonaCell™-HY杂交瘤试剂盒

ClonaCell™-HY Medium

ClonaCell™-HY Medium

ClonaCell™-HY Medium

ClonaCell™-HY Medium

ClonaCell™-HY Medium

ClonaCell™衔接挂钩

ClonaCell™-HY Medium D无HAT
产品手册
Cell and Gene Therapy: Products and Services for Your Research

产品类型：

Cell Culture Media and Supplements

品牌：

ImmunoCult，MethoCult，STEMdiff，StemSpan，mFreSR

产品号#：

产品名：
1:03:03

线上讲座
Predictive In Vitro Models for Toxicity Testing and Liver Research

发布日期: 5/9/23
30:53

线上讲座
Highly Characterized Human iPSCs and NPCs for Downstream Differentiation Applications

发布日期: 7/19/23
文献

Glover CH et al. (NOV 2006) PLoS computational biology 2 11 e158

Meta-analysis of differentiating mouse embryonic stem cell gene expression kinetics reveals early change of a small gene set.

Stem cell differentiation involves critical changes in gene expression. Identification of these should provide endpoints useful for optimizing stem cell propagation as well as potential clues about mechanisms governing stem cell maintenance. Here we describe the results of a new meta-analysis methodology applied to multiple gene expression datasets from three mouse embryonic stem cell (ESC) lines obtained at specific time points during the course of their differentiation into various lineages. We developed methods to identify genes with expression changes that correlated with the altered frequency of functionally defined,undifferentiated ESC in culture. In each dataset,we computed a novel statistical confidence measure for every gene which captured the certainty that a particular gene exhibited an expression pattern of interest within that dataset. This permitted a joint analysis of the datasets,despite the different experimental designs. Using a ranking scheme that favored genes exhibiting patterns of interest,we focused on the top 88 genes whose expression was consistently changed when ESC were induced to differentiate. Seven of these (103728at,8430410A17Rik,Klf2,Nr0b1,Sox2,Tcl1,and Zfp42) showed a rapid decrease in expression concurrent with a decrease in frequency of undifferentiated cells and remained predictive when evaluated in additional maintenance and differentiating protocols. Through a novel meta-analysis,this study identifies a small set of genes whose expression is useful for identifying changes in stem cell frequencies in cultures of mouse ESC. The methods and findings have broader applicability to understanding the regulation of self-renewal of other stem cell types. View Publication

产品类型：

产品号#：

06902

06952

00321

00322

00323

00324

00325

产品名：
挂图
Neural Stem Cells Overview of the types of NSCs and their potential use as therapeutic agents for disease
挂图
Regulatory T Cells Overview of the development, phenotype and functions of regulatory T cells
产品手册
Human Primary Cells

产品类型：

Primary and Cultured Cells

产品号#：

70031

70032

70033

70034

70035

70035.1

70035.2

70036

70037

70042

70046

70007

70007.1

70007.2

70013

70013.1

70013.2

70014

70015

70016

70017

70018

70019

70023

70023

产品名：

冻存的人外周血CD4+CD45RO+ T细胞

冻存的人外周血CD19+CD27- Naïve B细胞

冻存的人外周血CD19+ B细胞

冻存的人外周血单核细胞

冻存的人外周血CD14+单核细胞

冻存的人外周血CD14+单核细胞

冻存的人外周血CD14+单核细胞

#N/A

冻存的人外周血CD56+细胞

冻存的人外周血巨噬细胞

冻存的人外周血浆细胞样树突状细胞

冻存的人脐带血单核细胞

冻存的人脐带血单核细胞

冻存的人脐带血单核细胞

#N/A

#N/A

#N/A

冻存的人脐带血Pan T细胞

#N/A

#N/A

冻存的人脐带血CD4+CD45RA+ T细胞

冻存的人脐带血CD14+单核细胞

冻存的人脐带血CD56+细胞

#N/A

#N/A
研究综述
Pluripotent Stem Cells

产品类型：

细胞类型：

多能干细胞

产品号#：

产品名：
文献

Lannutti BJ et al. (FEB 2009) Blood 113 8 1778--85

Incomplete restoration of Mpl expression in the mpl-/- mouse produces partial correction of the stem cell-repopulating defect and paradoxical thrombocytosis.

Expression of Mpl is restricted to hematopoietic cells in the megakaryocyte lineage and to undifferentiated progenitors,where it initiates critical cell survival and proliferation signals after stimulation by its ligand,thrombopoietin (TPO). As a result,a deficiency in Mpl function in patients with congenital amegakaryocytic thrombocytopenia (CAMT) and in mpl(-/-) mice produces profound thrombocytopenia and a severe stem cell-repopulating defect. Gene therapy has the potential to correct the hematopoietic defects of CAMT by ectopic gene expression that restores normal Mpl receptor activity. We rescued the mpl(-/-) mouse with a transgenic vector expressing mpl from the promoter elements of the 2-kb region of DNA just proximal to the natural gene start site. Transgene rescued mice exhibit thrombocytosis but only partial correction of the stem cell defect. Furthermore,they show very low-level expression of Mpl on platelets and megakaryocytes,and the transgene-rescued megakaryocytes exhibit diminished TPO-dependent kinase phosphorylation and reduced platelet production in bone marrow chimeras. Thrombocytosis is an unexpected consequence of reduced Mpl expression and activity. However,impaired TPO homeostasis in the transgene-rescued mice produces elevated plasma TPO levels,which serves as an unchecked stimulus to drive the observed excessive megakaryocytopoiesis. View Publication

产品类型：

产品号#：

03434

03444

04960

04902

04900

04963

04962

04970

产品名：

MethoCult™GF M3434

MethoCult™GF M3434

MegaCult™-C胶原蛋白和不含细胞因子的培养基

胶原蛋白溶液

MegaCult™-C培养基无细胞因子

双室载玻片试剂盒

MegaCult™-C cfu染色试剂盒

MegaCult™-C不含细胞因子完整试剂盒