搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

产品手册

PancreaCult™ Organoid Growth Medium (Mouse)

产品类型：

品牌：

PancreaCult

产品号#：

06040

产品名：

PancreaCult™ 类器官生长培养基 (小鼠)

文献

Jeselsohn R et al. (JAN 2010) Cancer cell 17 1 65--76

Cyclin D1 kinase activity is required for the self-renewal of mammary stem and progenitor cells that are targets of MMTV-ErbB2 tumorigenesis.

Transplantation studies have demonstrated the existence of mammary progenitor cells with the ability to self-renew and regenerate a functional mammary gland. Although these progenitors are the likely targets for oncogenic transformation,correlating progenitor populations with certain oncogenic stimuli has been difficult. Cyclin D1 is required for lobuloalveolar development during pregnancy and lactation as well as MMTV-ErbB2- but not MMTV-Wnt1-mediated tumorigenesis. Using a kinase-deficient cyclin D1 mouse,we identified two functional mammary progenitor cell populations,one of which is the target of MMTV-ErbB2. Moreover,cyclin D1 activity is required for the self-renewal and differentiation of mammary progenitors because its abrogation leads to a failure to maintain the mammary epithelial regenerative potential and also results in defects in luminal lineage differentiation. View Publication

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产品名：

文献

Shirato K et al. ( 2017) Virology November 0--1

Wild-type human coronaviruses prefer cell-surface TMPRSS2 to endosomal cathepsins for cell entry

Human coronaviruses (HCoVs) enter cells via two distinct pathways: the endosomal pathway using cathepsins to activate spike protein and the cell-surface or early endosome pathway using extracellular proteases such as transmembrane protease serine 2 (TMPRSS2). We previously reported that clinical isolates of HCoV-229E preferred cell-surface TMPRSS2 to endosomal cathepsin for cell entry,and that they acquired the ability to use cathepsin L by repeated passage in cultured cells and were then able to enter cells via the endosomal pathway. Here,we show that clinical isolates of HCoV-OC43 and -HKU1 preferred the cell-surface TMRRSS2 to endosomal cathepsins for cell entry,similar to HCoV-229E. In addition,the cell-culture-adapted HCoV-OC43 lost the ability to infect and replicate in air-liquid interface cultures of human bronchial tracheal epithelial cells. These results suggest that circulating HCoVs in the field generally use cell-surface TMPRSS2 for cell entry,not endosomal cathepsins,in human airway epithelial cells. View Publication

产品类型：

产品号#：

05001

05021

05022

05008

产品名：

PneumaCult™-ALI 培养基

PneumaCult™-ALI 培养基含12 mm Transwell®插件

PneumaCult™-ALI 培养基含6.5 mm Transwell®插件

PneumaCult™交货中

技术公告

Mouse Hepatic Progenitor Organoid Culture: Supplementary Protocols

产品类型：

细胞类型：

上皮细胞，肝细胞

产品号#：

06030

产品名：

HepatiCult™ 类器官生长培养基 (小鼠)

技术公告

Mouse Pancreatic Exocrine Organoid Culture: Supplementary Protocols

产品类型：

细胞类型：

胰腺细胞

产品号#：

06040

产品名：

PancreaCult™ 类器官生长培养基 (小鼠)

产品手册

IntestiCult™ OGMM: Robust Growth of Mouse Intestinal Epithelial Organoids

产品类型：

品牌：

IntestiCult

产品号#：

06005

36254

产品名：

IntestiCult™ 肠道类器官生长培养基 (小鼠)

DMEM/F-12 with 15 mM HEPES

产品手册

ProstaCult™: Detect 7 Times More Mouse Prostate Epithelial Progenitors

产品类型：

品牌：

ProstaCult

产品号#：

07900

07901

07912

07913

78016

78016.1

78108.1

产品名：

DNase I 溶液（1 mg/mL）

胰蛋白酶-EDTA (0.25%)

胶原酶/透明质酸酶

Dispase（5 U/mL）

重组小鼠 EGF

重组小鼠FGF-21

Growth of Pancreatic Organoids in PancreaCult™

1:07

视频

Growth of Mouse Pancreatic Organoids in PancreaCult™ Culture Medium: Under a Microscope

发布日期: 4/18/18

文献

Charafe-Jauffret E et al. (JAN 2010) Clinical cancer research : an official journal of the American Association for Cancer Research 16 1 45--55

Aldehyde dehydrogenase 1-positive cancer stem cells mediate metastasis and poor clinical outcome in inflammatory breast cancer.

PURPOSE: To examine the role of cancer stem cells (CSC) in mediating metastasis in inflammatory breast cancer (IBC) and the association of these cells with patient outcome in this aggressive type of breast cancer. EXPERIMENTAL DESIGN: CSCs were isolated from SUM149 and MARY-X,an IBC cell line and primary xenograft,by virtue of increased aldehyde dehydrogenase (ALDH) activity as assessed by the ALDEFLUOR assay. Invasion and metastasis of CSC populations were assessed by in vitro and mouse xenograft assays. Expression of ALDH1 was determined on a retrospective series of 109 IBC patients and this was correlated with histoclinical data. All statistical tests were two sided. Log-rank tests using Kaplan-Meier analysis were used to determine the correlation of ALDH1 expression with development of metastasis and patient outcome. RESULTS: Both in vitro and xenograft assays showed that invasion and metastasis in IBC are mediated by a cellular component that displays ALDH activity. Furthermore,expression of ALDH1 in IBC was an independent predictive factor for early metastasis and decreased survival in this patient population. CONCLUSIONS: These results suggest that the metastatic,aggressive behavior of IBC may be mediated by a CSC component that displays ALDH enzymatic activity. ALDH1 expression represents the first independent prognostic marker to predict metastasis and poor patient outcome in IBC. The results illustrate how stem cell research can translate into clinical practice in the IBC field. View Publication

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产品号#：

01700

01705

01702

产品名：

ALDEFLUOR™ 试剂盒

ALDEFLUOR™ DEAB试剂

ALDEFLUOR™测定缓冲液

文献

Grudzien P et al. (OCT 2010) Anticancer research 30 10 3853--67

Inhibition of Notch signaling reduces the stem-like population of breast cancer cells and prevents mammosphere formation.

BACKGROUND: Cancer stem cells (CSCs) are believed to be responsible for breast cancer formation and recurrence; therefore,therapeutic strategies targeting CSCs must be developed. One approach may be targeting signaling pathways,like Notch,that are involved in stem cell self-renewal and survival. MATERIALS AND METHODS: Breast cancer stem-like cells derived from cell lines and patient samples were examined for Notch expression and activation. The effect of Notch inhibition on sphere formation,proliferation,and colony formation was determined. RESULTS: Breast cancer stem-like cells consistently expressed elevated Notch activation compared with bulk tumor cells. Blockade of Notch signaling using pharmacologic and genomic approaches prevented sphere formation,proliferation,and/or colony formation in soft agar. Interestingly,a gamma-secretase inhibitor,MRK003,induced apoptosis in these cells. CONCLUSION: Our findings support a crucial role for Notch signaling in maintenance of breast cancer stem-like cells,and suggest Notch inhibition may have clinical benefits in targeting CSCs. View Publication

产品类型：

产品号#：

01700

01705

产品名：

ALDEFLUOR™ 试剂盒

ALDEFLUOR™ DEAB试剂

产品手册

Reliable Antibodies for Your Research

产品类型：

品牌：

EasySep，RoboSep，RosetteSep

产品号#：

60100

60100.1

60100AD

60100AD.1

60104

60104AD.1

60104AZ

60104AZ.1

60104BT

60104BT.1

60104FI.1

60104PE

60104PE.1

60106

60106.1

60106AZ

60106AZ.1

60106BT

60106FI

60106FI.1

60106PE

60106PE.1

产品名：

抗β-微管蛋白III抗体，克隆AA10

抗β-微管蛋白III抗体，克隆AA10，Alexa Fluor® 488

抗β-微管蛋白III抗体，clone AA10，Alexa Fluor® 488

抗小鼠TCR Gamma/Delta抗体，clone GL3

抗小鼠TCR Gamma/Delta抗体，clone GL3，Alexa Fluor® 488

抗小鼠TCR Gamma/Delta抗体，clone GL3，APC

抗小鼠TCR Gamma/Delta抗体，clone GL3，Biotin

抗小鼠TCR Gamma/Delta抗体，clone GL3，FITC

抗小鼠TCR Gamma/Delta抗体，clone GL3，PE

抗人CD71（转铁蛋白受体）抗体，clone OKT9

抗人CD71（转铁蛋白受体）抗体，clone OKT9，APC

抗人CD71（转铁蛋白受体）抗体，clone OKT9，Biotin

抗人CD71（转铁蛋白受体）抗体，clone OKT9，FITC

抗人CD71（转铁蛋白受体）抗体，clone OKT9，PE

科学海报

HepatiCult™ for Human Hepatic Organoids: A Validated Culture System for Drug Toxicity Screening

产品类型：

产品号#：

产品名：

搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

Cyclin D1 kinase activity is required for the self-renewal of mammary stem and progenitor cells that are targets of MMTV-ErbB2 tumorigenesis.

Wild-type human coronaviruses prefer cell-surface TMPRSS2 to endosomal cathepsins for cell entry

Aldehyde dehydrogenase 1-positive cancer stem cells mediate metastasis and poor clinical outcome in inflammatory breast cancer.

Inhibition of Notch signaling reduces the stem-like population of breast cancer cells and prevents mammosphere formation.

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