搜索结果: 'methocult media formulations for human hematopoietic cells serum containing'

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Assays for Human Mammary Stem and Progenitor Cells Hierarchy of mammary epithelial cell differentiation and related assays

文献

Xaymardan M et al. (AUG 2009) Stem cells (Dayton,Ohio) 27 8 1911--20

c-Kit function is necessary for in vitro myogenic differentiation of bone marrow hematopoietic cells.

In recent years,the differentiation of bone marrow cells (BMCs) into myocytes has been extensively investigated,but the findings remain inconclusive. The purpose of this study was to determine the conditions necessary to induce myogenic differentiation in short-term cultures of adult BMCs,and to identify the BMC subpopulation responsible for this phenomenon. We report that high-density cultures of murine hematopoietic BMCs gave rise to spontaneous beating cell clusters in the presence of vascular endothelial and fibroblast growth factors. These clusters originated from c-kit(pos) cells. The formation of the clusters could be completely blocked by adding a c-kit/tyrosine kinase inhibitor,Gleevec (imatinib mesylate; Novartis International,Basel,Switzerland,http://www.novartis.com),to the culture. Cluster formation was also blunted in BMCs from c-kit-deficient (Kit(W)/Kit(W-v)) mice. Clustered cells expressed cardiomyocyte-specific transcription factor genes Gata-4 and Nkx2.5,sarcomeric proteins beta-MHC and MLC-2v,and ANF and connexin-43. Immunostaining revealed alpha-sarcomeric actinin expression in more than 90% of clustered cells. Under electron microscopy,the clustered cells exhibited a sarcomeric myofiber arrangement and z-bands. This study defines the microenvironment required to achieve a reproducible in vitro model of beating,myogenic cell clusters. This model could be used to examine the mechanisms responsible for the postnatal myogenic differentiation of BMCs. Our results identify c-kit(pos) bone marrow hematopoietic cells as the source of the myogenic clusters. View Publication

产品类型：

产品号#：

18757

18757RF

产品名：

EasySep™小鼠CD117（cKIT）正选试剂盒

RoboSep™ 小鼠CD117（cKIT）正选试剂盒含滤芯吸头

产品手册

Mammary Epithelial Cells: Standardized Media and Reagents

产品类型：

Cell Culture Media and Supplements

品牌：

ALDEFLUOR，EpiCult，MammoCult

产品号#：

01700

01705

01702

04902

05630

05601

05610

05620

07923

07070

07900

07901

07912

07913

07919

07980

36254

78104.1

78108.1

产品名：

ALDEFLUOR™ 试剂盒

ALDEFLUOR™ DEAB试剂

ALDEFLUOR™测定缓冲液

胶原蛋白溶液

EpiCult™-C 人培养基

EpiCult™-B 人培养基

EpiCult™-B 小鼠培养基试剂盒

MammoCult™人培养基试剂盒

Dispase (1 U/mL)

胭脂红明矾

DNase I 溶液（1 mg/mL）

胰蛋白酶-EDTA (0.25%)

胶原酶/透明质酸酶

Dispase（5 U/mL）

Gentle Collagenase/Hyaluronidase

肝素溶液

DMEM/F-12 with 15 mM HEPES

重组人双调蛋白

重组小鼠FGF-21

技术公告

A Sphere Culture Method for Mucociliary Differentiation of Primary Human Bronchial Epithelial Cells

产品类型：

Cell Culture Media and Supplements

细胞类型：

气道细胞

产品号#：

05001

05021

05022

05008

05040

00218CA.2

产品名：

PneumaCult™-ALI 培养基

PneumaCult™-ALI 培养基含12 mm Transwell®插件

PneumaCult™-ALI 培养基含6.5 mm Transwell®插件

PneumaCult™交货中

PneumaCult™-Ex Plus 培养基

人呼吸道上皮细胞课程 - CA - 秋季

文献

Eirew P et al. (DEC 2008) Nature medicine 14 12 1384--9

A method for quantifying normal human mammary epithelial stem cells with in vivo regenerative ability.

Previous studies have demonstrated that normal mouse mammary tissue contains a rare subset of mammary stem cells. We now describe a method for detecting an analogous subpopulation in normal human mammary tissue. Dissociated cells are suspended with fibroblasts in collagen gels,which are then implanted under the kidney capsule of hormone-treated immunodeficient mice. After 2-8 weeks,the gels contain bilayered mammary epithelial structures,including luminal and myoepithelial cells,their in vitro clonogenic progenitors and cells that produce similar structures in secondary transplants. The regenerated clonogenic progenitors provide an objective indicator of input mammary stem cell activity and allow the frequency and phenotype of these human mammary stem cells to be determined by limiting-dilution analysis. This new assay procedure sets the stage for investigations of mechanisms regulating normal human mammary stem cells (and possibly stem cells in other tissues) and their relationship to human cancer stem cell populations. View Publication

产品类型：

产品号#：

05601

产品名：

EpiCult™-B 人培养基

文献

Shirato K et al. ( 2017) Virology November 0--1

Wild-type human coronaviruses prefer cell-surface TMPRSS2 to endosomal cathepsins for cell entry

Human coronaviruses (HCoVs) enter cells via two distinct pathways: the endosomal pathway using cathepsins to activate spike protein and the cell-surface or early endosome pathway using extracellular proteases such as transmembrane protease serine 2 (TMPRSS2). We previously reported that clinical isolates of HCoV-229E preferred cell-surface TMPRSS2 to endosomal cathepsin for cell entry,and that they acquired the ability to use cathepsin L by repeated passage in cultured cells and were then able to enter cells via the endosomal pathway. Here,we show that clinical isolates of HCoV-OC43 and -HKU1 preferred the cell-surface TMRRSS2 to endosomal cathepsins for cell entry,similar to HCoV-229E. In addition,the cell-culture-adapted HCoV-OC43 lost the ability to infect and replicate in air-liquid interface cultures of human bronchial tracheal epithelial cells. These results suggest that circulating HCoVs in the field generally use cell-surface TMPRSS2 for cell entry,not endosomal cathepsins,in human airway epithelial cells. View Publication

产品类型：

产品号#：

05001

05021

05022

05008

产品名：

PneumaCult™-ALI 培养基

PneumaCult™-ALI 培养基含12 mm Transwell®插件

PneumaCult™-ALI 培养基含6.5 mm Transwell®插件

PneumaCult™交货中

科学海报

HepatiCult™ for Human Hepatic Organoids: A Validated Culture System for Drug Toxicity Screening

产品类型：

Cell Culture Media and Supplements

产品号#：

产品名：

技术公告

Supplementary Protocols for IntestiCult™ Organoid Growth Medium (Human)

产品类型：

Cell Culture Media and Supplements

细胞类型：

上皮细胞，肠道细胞

产品号#：

06010

产品名：

IntestiCult™ 类器官生长培养基 (人)

产品手册

PneumaCult™ Culture System for Airway Epithelial Cells

产品类型：

Cell Culture Media and Supplements

品牌：

PneumaCult

产品号#：

05001

05021

05022

05008

60154

78104.1

产品名：

PneumaCult™-ALI 培养基

PneumaCult™-ALI 培养基含12 mm Transwell®插件

PneumaCult™-ALI 培养基含6.5 mm Transwell®插件

PneumaCult™交货中

抗人p63（deltaN）抗体，克隆Poly6190

重组人双调蛋白

Using Human Pluripotent Stem Cell-Derived Neural Organoids for Disease Modeling

31:49

线上讲座

Using Human Pluripotent Stem Cell-Derived Neural Organoids for Disease Modeling

发布日期: 8/1/24

科学海报

Human Intestinal Organoid Culture System for Drug-Induced Gastrointestinal Toxicity Screening

产品类型：

Cell Culture Media and Supplements

Conference：

SOT 2023

产品号#：

产品名：

文献

Petersen OW and Polyak K (MAY 2010) Cold Spring Harbor perspectives in biology 2 5 a003160

Stem cells in the human breast.

The origins of the epithelial cells participating in the development,tissue homeostasis,and cancer of the human breast are poorly understood. However,emerging evidence suggests a role for adult tissue-specific stem cells in these processes. In a hierarchical manner,these generate the two main mammary cell lineages,producing an increasing number of cells with distinct properties. Understanding the biological characteristics of human breast stem cells and their progeny is crucial in attempts to compare the features of normal stem cells and cancer precursor cells and distinguish these from nonprecursor cells and cells from the bulk of a tumor. A historical overview of research on human breast stem cells in primary tissue and in culture reveals the progress that has been made in this area,whereas a focus on the cell-of-origin and reprogramming that occurs during neoplastic conversion provides insight into the enigmatic way in which human breast cancers are skewed toward the luminal epithelial lineage. View Publication

产品类型：

产品号#：

01700

01705

产品名：

ALDEFLUOR™ 试剂盒

ALDEFLUOR™ DEAB试剂

搜索结果: 'methocult media formulations for human hematopoietic cells serum containing'

c-Kit function is necessary for in vitro myogenic differentiation of bone marrow hematopoietic cells.

A method for quantifying normal human mammary epithelial stem cells with in vivo regenerative ability.

Wild-type human coronaviruses prefer cell-surface TMPRSS2 to endosomal cathepsins for cell entry

Stem cells in the human breast.

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