搜索结果: 'methocult media formulations for human hematopoietic cells serum containing'

STEMvision™ 用于造血集落形成单位（CFU）测定的自动化和标准化集落计数
文献

Lam AC et al. (DEC 2001) Transfusion 41 12 1567--76

Preclinical ex vivo expansion of cord blood hematopoietic stem and progenitor cells: duration of culture; the media, serum supplements, and growth factors used; and engraftment in NOD/SCID mice.

BACKGROUND: Ex vivo expansion of cord blood (CB) hematopoietic stem and progenitor cells increases cell dose and may reduce the severity and duration of neutropenia and thrombocytopenia after transplantation. This study's purpose was to establish a clinically applicable culture system by investigating the use of cytokines,serum-free media,and autologous plasma for the expansion of CB cells and the engraftment of expanded product in nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice. STUDY DESIGN AND METHODS: Enriched CB CD34+ cells were cultured in four media (Iscove's modified Dulbecco's medium with FCS,Gibco; X-Vivo-10,BioWhittaker; QBSF-60,Quality Biological; and StemSpan SFEM,Stem Cell Technologies) with four cytokine combinations (thrombopoietin [TPO],SCF,Flt-3 ligand [FL] with and without G-CSF,and/or IL-6). The effect of autologous CB plasma was also investigated. The read-out measures were evaluated on Days 8 and 12. After expansion at the optimized condition,cultured cells were transplanted into sublethally irradiated NOD/SCID mice. The engraftment of human CD45+ cells and subsets in the bone marrow,spleen,and peripheral blood was determined. RESULTS: QBSF-60 or StemSpan SFEM supported high yields of early progenitors (CD34+ cells,textlessor= 64.8-fold; CD34+CD38- cells,330-fold; CFU-granulocyte erythroid macrophage megakaryocyte [GEMM],248-fold) and CFUs of the myeloid (CFU-GM,407-fold) and erythroid (BFU/CFU-E,144-fold) lineages. The expansion of the megakaryocytic lineage was consistently higher in X-Vivo-10 (CFU-megakaryocyte,684-fold). Autologous plasma promoted colony formation but reduced CD34+ cells and CFU-GEMM. The addition of G-CSF or IL-6 improved cell yields; G-CSF was more effective for committed progenitors. Expansion products from cultures in QBSF-60 with the cytokines engrafted and differentiated into the myeloid and lymphoid lineages in NOD/SCID mice. CONCLUSION: The data supported the strategy of expansion. The optimized condition may be applicable to clinical expansion for the abrogation or reduction of posttransplant cytopenia. View Publication

产品类型：

产品号#：

09600

09650

产品名：

StemSpan™ SFEM

StemSpan™ SFEM
技术手册
Human Colony-Forming Unit (CFU) Assays Using MethoCult™

产品类型：

Cell Culture Media and Supplements

产品号#：

04436

04064

04100

04230

04236

04431

04434

04444

04464

04531

04535

04545

04536

04564

09300

04035

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00215US.4

产品名：

MethoCult™ SF H4436

MethoCult™ H4034 Optimum启动试剂盒套装

MethoCult™ H4100

MethoCult™H4230

MethoCult™SF H4236

MethoCult™H4431

MethoCult™H4434经典

MethoCult™H4434经典

MethoCult™ H4434 Classic启动试剂盒套装

MethoCult™H4531

MethoCult™H4535富集无EPO

MethoCult™ H4535 Enriched，不含EPO

MethoCult™ SF H4536

入门套件MethoCult™H4534经典无EPO

含有10% 牛血清白蛋白（BSA）的 Iscove's MDM

MethoCult™H4035 Optimum无EPO

MethoCult™H4330

MethoCult™H4034 Optimum

MethoCult™H4034 Optimum

MethoCult™H4435富集

MethoCult™H4435富集

MethoCult™H4534经典无EPO

MethoCult™H4534经典无EPO

MethoCult™表达

MethoCult™表达

造血祖细胞检测标准化培训课程
人冷冻脐带血CFU测定熟练度测试项目 用于造血集落形成单位（CFU）测定
产品手册
Hematopoietic Stem and Progenitor Cells - Products for Your Research

产品类型：

Cell Culture Media and Supplements

品牌：

ALDEFLUOR，MethoCult，MyeloCult，STEMdiff，STEMvision，SmartDish，StemSpan，ThawSTAR

产品号#：

00650

00651

01700

01705

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02690

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03534

03630

03134

03231

03234

03334

03434

03444

03436

04436

04064

04100

04230

产品名：

人骨髓质量控制试剂盒

人脐带血质量控制试剂盒

ALDEFLUOR™ 试剂盒

ALDEFLUOR™ DEAB试剂

ALDEFLUOR™测定缓冲液

琼脂白细胞条件培养基

StemSpan™CC100

StemSpan™CD34+扩增补充（10X）

StemSpan™髓系扩增补品（100X）

StemSpan™巨核细胞扩增补充（100X）

StemSpan™CC110

人类低密度脂蛋白

MethoCult™GF M3534

MethoCult™M3630

MethoCult™M3134

MethoCult™M3231

MethoCult™M3234

MethoCult™M3334

MethoCult™GF M3434

MethoCult™GF M3434

MethoCult™SF M3436

MethoCult™ SF H4436

MethoCult™ H4034 Optimum启动试剂盒套装

MethoCult™ H4100

MethoCult™H4230
53:36

线上讲座
Assays for Hematopoietic Stem and Progenitor Cells in Cord Blood

发布日期: 5/10/10
53:35

线上讲座
Optimization and Standardization of the Colony-Forming Unit Assay for Hematopoietic Progenitor Cells

发布日期: 9/16/10
产品手册
STEMvision™ Automated and Standardized Counting of CFU Assays of Human Hematopoietic Cells

产品类型：

Instruments and Software

品牌：

STEMvision

产品号#：

04035

04034

04044

04437

04447

22001

22005

22006

22007

22008

22009

22011

22012

27370

27371

产品名：

MethoCult™H4035 Optimum无EPO

MethoCult™H4034 Optimum

MethoCult™H4034 Optimum

MethoCult™表达

MethoCult™表达

STEMvision™ 人脐带血7-天CFU分析包

STEMvision™ 彩色人脐带血14-天CFU分析包

STEMvision™ 彩色人骨髓14-天CFU分析包

STEMvision™ 彩色人动员外周血14-天CFU分析包

STEMvision™ 小鼠总CFU分析包

STEMvision™ 小鼠髓系CFU分析包

STEMvision™ 小鼠红系CFU分析包

STEMvision™ 小鼠CFU分析包（髓系和红系）

SmartDish™ - 5个

SmartDish™ - 50个
点播
Human Hematopoietic CFU Assay Course Learn how to perform the CFU assay with the CFU Assay Starter Kit.
文献

Zhang CC et al. (APR 2008) Blood 111 7 3415--23

Angiopoietin-like 5 and IGFBP2 stimulate ex vivo expansion of human cord blood hematopoietic stem cells as assayed by NOD/SCID transplantation.

Hematopoietic stem cells (HSCs) are the basis of bone marrow transplantation and are attractive target cells for hematopoietic gene therapy,but these important clinical applications have been severely hampered by difficulties in ex vivo expansion of HSCs. In particular,the use of cord blood for adult transplantation is greatly limited by the number of HSCs. Previously we identified angiopoietin-like proteins and IGF-binding protein 2 (IGFBP2) as new hormones that,together with other factors,can expand mouse bone marrow HSCs in culture. Here,we measure the activity of multipotent human severe combined immunodeficient (SCID)-repopulating cells (SRCs) by transplantation into the nonobese diabetic SCID (NOD/SCID) mice; secondary transplantation was performed to evaluate the self-renewal potential of SRCs. A serum-free medium containing SCF,TPO,and FGF-1 or Flt3-L cannot significantly support expansion of the SRCs present in human cord blood CD133+ cells. Addition of either angiopoietin-like 5 or IGF-binding protein 2 to the cultures led to a sizable expansion of HSC numbers,as assayed by NOD/SCID transplantation. A serum-free culture containing SCF,TPO,FGF-1,angiopoietin-like 5,and IGFBP2 supports an approximately 20-fold net expansion of repopulating human cord blood HSCs,a number potentially applicable to several clinical processes including HSC transplantation. View Publication

产品类型：

产品号#：

09600

09650

28600

产品名：

StemSpan™ SFEM

StemSpan™ SFEM

L-Calc™有限稀释软件
文献

Yasui K et al. (JAN 2003) Stem cells (Dayton,Ohio) 21 2 143--51

Differences between peripheral blood and cord blood in the kinetics of lineage-restricted hematopoietic cells: implications for delayed platelet recovery following cord blood transplantation.

Cord blood (CB) cells are a useful source of hematopoietic cells for transplantation. The hematopoietic activities of CB cells are different from those of bone marrow and peripheral blood (PB) cells. Platelet recovery is significantly slower after transplantation with CB cells than with cells from other sources. However,the cellular mechanisms underlying these differences have not been elucidated. We compared the surface marker expression profiles of PB and CB hematopoietic cells. We focused on two surface markers of hematopoietic cell immaturity,i.e.,CD34 and AC133. In addition to differences in surface marker expression,the PB and CB cells showed nonidentical differentiation pathways from AC133(+)CD34(+) (immature) hematopoietic cells to terminally differentiated cells. The majority of the AC133(+)CD34(+) PB cells initially lost AC133 expression and eventually became AC133(-)CD34(-) cells. In contrast,the AC133(+)CD34(+) CB cells did not go through the intermediate AC133(-)CD34(+) stage and lost both markers simultaneously. Meanwhile,the vast majority of megakaryocyte progenitors were of the AC133(-)CD34(+) phenotype. We conclude that the delayed recovery of platelets after CB transplantation is due to both subpopulation distribution and the process of differentiation from AC133(+)CD34(+) cells. View Publication

产品类型：

产品号#：

04064

04960

04902

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04961

04901

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产品名：

MethoCult™ H4034 Optimum启动试剂盒套装

MegaCult™-C胶原蛋白和不含细胞因子的培养基

胶原蛋白溶液

MegaCult™-C培养基无细胞因子

MegaCult™-C胶原蛋白和细胞因子培养基

MegaCult™-C细胞因子培养基

双室载玻片试剂盒

MegaCult™-C cfu染色试剂盒

MegaCult™-C不含细胞因子完整试剂盒

MegaCult™-C细胞因子完整试剂盒
科学海报
A Xeno-Free Culture System for Efficient Derivation and Amplification of Human Endothelial Colony-Forming Cells from Umbilical Cord Blood

产品类型：

Cell Culture Media and Supplements

Conference：

ARVO 2019

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