搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

抗小鼠CD44抗体，clone IM7 抗人、鼠、恒河猴CD44（组织非特异性碱性磷酸酶）的大鼠Monoclonal IgG2b抗体
抗小鼠CD117抗体，clone 2B8 大鼠Monoclonal IgG2b抗体，抗小鼠CD117（c-Kit）
抗小鼠Sca1抗体，clone E13-161.7 大鼠抗小鼠Sca1 (Ly-6A/E)Monoclonal IgG2a抗体
抗小鼠EPCR抗体，clone RMEPCR1560（1560） 抗小鼠EPCR（CD201）大鼠Monoclonal IgG2b抗体
抗小鼠OCT4（OCT3）抗体，clone 40 抗人、鼠OCT4（OCT3）的小鼠Monoclonal IgG1抗体
产品手册
MethoCult™ Media for Performing Hematopoietic Colony-Forming Unit (CFU) Assays

产品类型：

品牌：

MethoCult

产品号#：

00217

00217UK

00215

00215GER

00215UK

03534

03630

03134

03231

03234

03334

03434

03444

03436

04436

04064

04100

04230

04236

04431

04434

04444

04464

04531

04535

04545

04536

04564

07700

07800

07850

27100

27150

27140

27141

27500

38068

28110

28120

28230

28240

03

产品名：

MethoCult™ GF M3534

MethoCult™ M3630

MethoCult™ M3134

MethoCult™ M3231

MethoCult™ M3234

MethoCult™ M3334

MethoCult™ GF M3434

MethoCult™ GF M3434

MethoCult™ SF M3436

MethoCult™ SF H4436

MethoCult™ H4034 Optimum 入门试剂盒

MethoCult™ H4100

MethoCult™ H4230

MethoCult™ SF H4236

MethoCult™ H4431

MethoCult™ H4434 Classic

MethoCult™ H4434 Classic

MethoCult™ H4434 Classic 套装

MethoCult™ H4531

MethoCult™ H4535 Enriched，不含EPO

MethoCult™ H4535 Enriched，不含EPO

MethoCult™ SF H4536

MethoCult™ H4534 Classic 无 EPO 入门试剂盒

含 2% FBS的Iscove's MDM

氯化铵溶液

氯化铵溶液

35 mm培养皿

35 mm培养皿

Corning® 60 mm 带网格培养皿

16号钝针

16号钝针

3 mL注射器

3 mL注射器
技术公告
MethoCult™ Formulation Optimized for Mouse BFU-E Detection

产品类型：

细胞类型：

造血干祖细胞

产品号#：

00217

00217UK

03231

03234

03334

03434

03444

03436

03236

00217CA.3

00217UK.3

产品名：

MethoCult™ M3231

MethoCult™ M3234

MethoCult™ M3334

MethoCult™ GF M3434

MethoCult™ GF M3434

MethoCult™ SF M3436

MethoCult™ SF M3236

发布日期: 11/01/2009
Limó et al. (NOV 1997) Blood 90 9 3316--21

High-titer retroviral vectors containing the enhanced green fluorescent protein gene for efficient expression in hematopoietic cells.

Retroviral vectors constitute the most efficient system to deliver and integrate foreign genes into mammalian cells. We have developed a producer cell line that yields high titers of amphotropic retroviral vectors carrying the enhanced green fluorescent protein (EGFP) gene,a codon humanized,red-shifted variant of the green fluorescent protein (GFP) gene,which can be used as a selectable marker. We have used a hybrid vector that has been shown to efficiently drive gene expression in hematopoietic cells. Virtually all murine and human cell lines and primary human hematopoietic cells tested were transduced with varying efficiency after incubation with vector-containing supernatants. Human CD34(+) cells obtained from cord blood or aphereses products were transduced using a protocol that involves daily addition of vector-containing supernatants for 6 consecutive days. At day 6,up to 16% of the cells expressed EGFP,as assessed by flow cytometry. Sorted EGFP-expressing cells were able to produce fluorescent hematopoietic colonies. EGFP's main advantages are its fast flow cytometry determination and the possibility of cell sorting and simultaneous evaluation of the transduction efficiency along with other phenotypic markers. View Publication

产品类型：

产品号#：

04034

04044

产品名：

MethoCult™ H4034 Optimum

MethoCult™ H4034 Optimum
技术公告
Flow Cytometry Methods for Identifying Mouse Hematopoietic Stem and Progenitor Cells

产品类型：

细胞类型：

造血干祖细胞

产品号#：

产品名：

发布日期: 08/31/2018
Lu S-J et al. (JUL 2013) Regenerative medicine 8 4 413--424

3D microcarrier system for efficient differentiation of human pluripotent stem cells into hematopoietic cells without feeders and serum [corrected].

BACKGROUND Human embryonic stem cells (hESCs) have been derived and maintained on mouse embryonic fibroblast feeders to keep their undifferentiated status. To realize their clinical potential,a feeder-free and scalable system for large scale production of hESCs and their differentiated derivatives is required. MATERIALS & METHODS hESCs were cultured and passaged on serum/feeder-free 3D microcarriers for five passages. For embryoid body (EB) formation and hemangioblast differentiation,the medium for 3D microcarriers was directly switched to EB medium. RESULTS hESCs on 3D microcarriers maintained pluripotency and formed EBs,which were ten-times more efficient than hESCs cultured under 2D feeder-free conditions (0.11 ± 0.03 EB cells/hESC input 2D vs 1.19 ± 0.32 EB cells/hESC input 3D). After replating,EB cells from 3D culture readily developed into hemangioblasts with the potential to differentiate into hematopoietic and endothelial cells. Furthermore,this 3D system can also be adapted to human induced pluripotent stem cells,which generate functional hemangioblasts with high efficiency. CONCLUSION This 3D serum- and stromal-free microcarrier system is important for future clinical applications,with the potential of developing to a GMP-compatible scalable system. View Publication

产品类型：

产品号#：

04436

05850

05857

05870

05875

85850

85857

85870

85875

产品名：

MethoCult™ SF H4436

mTeSR™1

mTeSR™1
Heckl D et al. (APR 2011) Blood 117 14 3737--47

Lentiviral gene transfer regenerates hematopoietic stem cells in a mouse model for Mpl-deficient aplastic anemia.

Thpo/Mpl signaling plays an important role in the maintenance of hematopoietic stem cells (HSCs) in addition to its role in megakaryopoiesis. Patients with inactivating mutations in Mpl develop thrombocytopenia and aplastic anemia because of progressive loss of HSCs. Yet,it is unknown whether this loss of HSCs is an irreversible process. In this study,we used the Mpl knockout (Mpl(-/-)) mouse model and expressed Mpl from newly developed lentiviral vectors specifically in the physiologic Mpl target populations,namely,HSCs and megakaryocytes. After validating lineage-specific expression in vivo using lentiviral eGFP reporter vectors,we performed bone marrow transplantation of transduced Mpl(-/-) bone marrow cells into Mpl(-/-) mice. We show that restoration of Mpl expression from transcriptionally targeted vectors prevents lethal adverse reactions of ectopic Mpl expression,replenishes the HSC pool,restores stem cell properties,and corrects platelet production. In some mice,megakaryocyte counts were atypically high,accompanied by bone neo-formation and marrow fibrosis. Gene-corrected Mpl(-/-) cells had increased long-term repopulating potential,with a marked increase in lineage(-)Sca1(+)cKit(+) cells and early progenitor populations in reconstituted mice. Transcriptome analysis of lineage(-)Sca1(+)cKit(+) cells in Mpl-corrected mice showed functional adjustment of genes involved in HSC self-renewal. View Publication

产品类型：

产品号#：

09600

09650

产品名：

StemSpan™ SFEM

StemSpan™ SFEM
产品手册
StemSpan™: Defined Media and Supplements for Hematopoietic Cell Expansion

产品类型：

品牌：

StemSpan

产品号#：

02690

02691

02693

02696

02697

02692

09600

09650

09605

09655

09800

09850

09805

09855

09900

09910

09920

产品名：

StemSpan™ CC100

StemSpan™ CD34+扩增添加物 (10X)

StemSpan™髓系扩增添加物 (100X)

StemSpan™巨核细胞扩增添加物 (100X)

StemSpan™ CC110

StemSpan™红系扩增添加物 (100X)

StemSpan™ SFEM

StemSpan™ SFEM

StemSpan™ SFEM II

StemSpan™ SFEM II

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搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

High-titer retroviral vectors containing the enhanced green fluorescent protein gene for efficient expression in hematopoietic cells.

3D microcarrier system for efficient differentiation of human pluripotent stem cells into hematopoietic cells without feeders and serum [corrected].

Lentiviral gene transfer regenerates hematopoietic stem cells in a mouse model for Mpl-deficient aplastic anemia.

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